Silencing of Entamoeba histolytica Glucosamine 6-Phosphate Isomerase by RNA Interference Inhibits the Formation of Cyst-Like Structures

Table 2

Effect of Gln6Pi knockdown by a mix of si 154-Gln6Pi and si 229-Gln6Pi siRNAs on viability, conversion rate and chitin expression of trophozoites induced to CLS.

(siRNA mix)	Interference trophozoites viability (FDA); (%  ± S.D.)	Treatment solution^a/incubation time	Conversion rate; (%  ± S.D.)^b	Viability; (FDA); (%  ± S.D.)	Staining calcofluor white; (%  ± S.D.)
5 μg	95 ± 3.6	4 mM/6 h	27 ± 4.4	69 ± 5	95 ± 2
10 μg	91 ± 1.5	4 mM/6 h	19 ± 5.3	65 ± 4.5	90 ± 8
20 μg	90 ± 2.3	4 mM/6 h	9 ± 3	36 ± 1.1	65 ± 2
40 μg	87 ± 3	4 mM/6 h	5 ± 2.1	12 ± 2.8	15 ± 4.8

^aHydrogen peroxide 30% containing traces of several dications, see Section  2.2.
^bThree independent experiments were done by triplicate.
Conversion rate: percentage of cells that were resistant to 0.5% sarkosyl.
FDA: fluorescein diacetate.
S.D.: standard deviation.