Table 2: Effect of Gln6Pi knockdown by a mix of si 154-Gln6Pi and si 229-Gln6Pi siRNAs on viability, conversion rate and chitin expression of trophozoites induced to CLS.

(siRNA mix)Interference trophozoites viability (FDA)
(%  ± S.D.)
Treatment solutiona/incubation time Conversion rate
(%  ± S.D.)b
Viability
(FDA)
(%  ± S.D.)
Staining calcofluor white
(%  ± S.D.)

5 μg95 ± 3.64 mM/6 h27 ± 4.469 ± 5 95 ± 2
10 μg91 ± 1.54 mM/6 h19 ± 5.365 ± 4.590 ± 8
20 μg90 ± 2.34 mM/6 h9 ± 336 ± 1.165 ± 2
40 μg87 ± 34 mM/6 h5 ± 2.112 ± 2.815 ± 4.8

aHydrogen peroxide 30% containing traces of several dications, see Section  2.2.
bThree independent experiments were done by triplicate.
Conversion rate: percentage of cells that were resistant to 0.5% sarkosyl.
FDA: fluorescein diacetate.
S.D.: standard deviation.