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Figure 2: Human hepatocellular carcinoma cell line characterization. (a) Assessment of EGFR expression in HCC cell lines, including Hep3B, PLC/PRF/5, and HepG2 cells. Protein ladders are indicated in kDa. Bands for EGFR and β-actin are indicated by arrowheads. (b) EGFR expression in HCC cell lines at mRNA level was assessed by quantitative real-time PCR. Relative quantification of EGFR RNA expression was validated by 18S RNA. (c) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 cells using FITC labeled anti-EGFR antibody. Positive staining of EGFR was shown in red. Nuclei staining using DAPI is shown in blue. (d) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 cells using Alexa680- . Staining using unlabeled Ac-Cys- as a blocking reagent was also shown. Positive staining of EGFR was shown in red. Nuclei staining using DAPI is shown in blue.