Figure 3: Micrographs of double-immunofluorescence staining reveal the localization of perlecan and laminin ((a)–(c)) or type IV collagen ((d)–(f)) in palate of a mouse embryo at E15.5. ((a), (d)) Perlecan reactivity was evident in the basement membrane of oral palate epithelium (arrows), blood vessels, and some stroma () in palate. ((b), (e)) Weak or discontinuous laminin (b) or type IV collagen (e) immunoreactivity was evident in the basement membrane of the MES (arrowheads). ((c), (f)) Colocalization of perlecan and laminin (c) or type IV collagen (f) was observed in the basement membrane of oral epithelium and blood vessels. Some parts of the basement membrane of the MES had only laminin (c) or type IV collagen (f) reactivity (arrowheads). Adjacent sections. P, palate; O, oropharynx; N, nasal septum; BV, blood vessels; MES, medial epithelial seam. Bars: 25 μm.