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BioMed Research International
Volume 2013 (2013), Article ID 760681, 11 pages
http://dx.doi.org/10.1155/2013/760681
Research Article

Molecular Identification and Ultrastructural and Phylogenetic Studies of Cyanobacteria from Association with the White Sea Hydroid Dynamena pumila (L., 1758)

1Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, Leninskie Gory 1-40, Moscow 119992, Russia
2Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia
3Lomonosov Moscow State University, Faculty of Biology, Leninskie Gory 1-12, Moscow 119991, Russia
4N.I.Vavilov Institute of General Genetics, Russian Academy of Science, Gubkin Street 3, Moscow 119333, Russia
5N.K.Kol’tsov Institute of Developmental Biology, Russian Academy of Science, Vavilova Street 26, Moscow 119334, Russia

Received 28 February 2013; Accepted 22 April 2013

Academic Editor: Vassily Lyubetsky

Copyright © 2013 O. A. Koksharova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Three new cyanobacterial strains, that have been previously purified from the hydroid Dynamena pumila (L., 1758), isolated from the White Sea, were studied using scanning and transmission electron microscopy methods and were characterized by using almost complete sequence of the 16S rRNA gene, internal transcribed spacer 16S-23S rRNA, and part of the gene for 23S rRNA. The full nucleotide sequences of the rRNA gene clusters were deposited to GenBank (HM064496.1, GU265558.1, JQ259187.1). Comparison of rRNA gene cluster sequences of Synechococcus cyanobacterium 1Dp66E-1, Oscillatoriales cyanobacterium 2Dp86E, and Nostoc sp. 10Dp66E with all sequences present at the GenBank shows that these cyanobacterial strains do not have 100% identity with any organisms investigated previously. Furthermore, for the first time heterotrophic bacterium, associated with Nostoc sp. 10Dp66E, was identified as a member of the new phylum Gemmatimonadetes, genus of Gemmatimonas (GenBank accession number is JX437625.1). Phylogenetic analysis showed that cyanobacterium Synechococcus sp. 1Dp66E-1 forms the unique branch and belongs to a cluster of Synechococcus, including freshwater and sea strains. Oscillatoriales cyanobacterium 2Dp86E belongs to a cluster of Leptolyngbya strains. Isolate Nostoc sp. 10Dp66E forms unique branch and belongs to a cluster of the genus Nostoc, with the closest relative of Nostoc commune isolates.