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BioMed Research International
Volume 2013 (2013), Article ID 760904, 8 pages
http://dx.doi.org/10.1155/2013/760904
Research Article

Gene Expression Profiling of Clostridium botulinum under Heat Shock Stress

1College of Life Science, Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou 325000, China
2School of Basic Medical Science, Wenzhou Medical University, Wenzhou 325000, China
3Neurosurgery, The First Hospital of Jiamusi University, Jiamusi 154007, China
4Cardiovascular Surgery, The Fourth Hospital of Harbin Medical University, Harbin 157003, China
5Clinical Medicine Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China

Received 28 May 2013; Revised 16 August 2013; Accepted 25 August 2013

Academic Editor: Gulam Waris

Copyright © 2013 Wan-dong Liang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

During growth, C. botulinum is always exposed to different environmental changes, such as temperature increase, nutrient deprivation, and pH change; however, its corresponding global transcriptional profile is uncharacterized. This study is the first description of the genome-wide gene expression profile of C. botulinum in response to heat shock stress. Under heat stress (temperature shift from 37°C to 45°C over a period of 15 min), 176 C. botulinum ATCC 3502 genes were differentially expressed. The response included overexpression of heat shock protein genes (dnaK operon, groESL, hsp20, and htpG) and downregulation of aminoacyl-tRNA synthetase genes (valS, queA, tyrR, and gatAB) and ribosomal and cell division protein genes (ftsZ and ftsH). In parallel, several transcriptional regulators (marR, merR, and ompR families) were induced, suggesting their involvement in reshuffling of the gene expression profile. In addition, many ABC transporters (oligopeptide transport system), energy production and conversion related genes (glpA and hupL), cell wall and membrane biogenesis related genes (fabZ, fabF, and fabG), flagella-associated genes (flhA, flhM, flhJ, flhS, and motAB), and hypothetical genes also showed changed expression patterns, indicating that they may play important roles in survival under high temperatures.