Gene Expression Profiling of Clostridium botulinum under Heat Shock Stress

Table 1

The top 40 differentially expressed genes in response to heat shock stress in Clostridium botulinum ATCC 3502.

Locus tag	COG	Gene name	Function class	Fold change	Function description
Posttranslational modification, protein turnover, and chaperones [O]
CBO2958	COG0484	dnaJ	[O]	+8.9	Chaperone protein
CBO2959	COG0443	dnaK	[O]	+17.2	Chaperone protein
CBO0189	COG0484		[O]	+8.2	DnaJ-related protein
CBO2960	COG0576	grpE	[O]	+5.6	Heat shock protein
CBO3299	COG0234	groES	[O]	+14.2	10 kDa chaperonin GroES
CBO3298	COG0459	groEL	[O]	+8.3	60 kDa chaperonin GroEL
CBO0501	COG2077	tpx	[O]	+5.0	Thiol peroxidase
CBO0803		ha-33		+1.8	Toxin cluster gene ha-33
CBO0802		ha-17		+1.6	Toxin cluster gene ha-17
Transcription [K]
CBO0645	COG1846		[K]	+16.1	MarR-family transcriptional regulator
CBO1012	COG1846		[K]	+7.7	MarR-family transcriptional regulator
CBO1017	COG4978		[KT]	−5.8	MerR-family transcriptional regulator
CBO2304	COG1695		[K]	+5.8	Transcriptional regulator, PadR family
CBO0786	COG0745		[KT]	+4.4	Two component response regulator
CBO0100	COG1609	ccpA	[K]	−7.3	LacI-family transcriptional regulator
CBO2938	COG0568	rpoD	[K]	+4.2	RNA polymerase sigma factor (sigma-43)
CBO3519	COG0782	greA	[K]	−5.0	Transcription elongation factor
Translation and ribosomal structure and biogenesis [J]
CBO3164	COG0525	valS	[J]	−12.1	Valyl-tRNA synthetase
CBO3069	COG0809	queA	[J]	−9.2	tRNA ribosyltransferase-isomerase
CBO1204	COG0162	tyrR	[J]	−5.6	Tyrosyl-tRNA synthetase
CBO3265	COG0064	gatB	[J]	−4.3	Glutamyl-tRNA amidotransferase subunit B
CBO3503	COG0442	proS2	[J]	−4.3	Prolyl-tRNA synthetase
CBO3323	COG0162	tyrS	[J]	−4.7	Tyrosyl-tRNA synthetase
CBO3470	COG0198	rplX	[J]	−6.7	50S ribosomal protein L24
CBO3474	COG0092	rpsC	[J]	−4.6	30S ribosomal protein S3
CBO0400	COG0465	ftsH	[J]	−6.3	Cell division protein
Amino acid transport and metabolism [E]
CBO1675	COG0747		[E]	+6.8	Oligopeptide-binding protein
CBO1860	COG0697	ecsC	[E]	+4.1	Membrane protein
Cell wall, membrane, and envelope biogenesis [M]
CBO2101	COG1087		[M]	−4.2	UDP-glucose epimerase
CBO3597	COG0764	fabZ	[M]	+8.3	(3R)-Hydroxymyristoyl-ACP dehydratase
Hypothetical proteins
CBO3522				−4.1	Hypothetical protein
CBO2185				+5.3	Hypothetical protein
CBO2640				−6.3	Hypothetical protein
CBO2096				+5.8	Hypothetical protein
CBO3353				+7.6	Hypothetical protein
Function unknown [S]
CBO1070	COG3862		[S]	+6.0	Hypothetical protein
CBO0014	COG3862		[S]	−4.2	Hypothetical protein
CBO2935	COG0327		[S]	+4.1	Hypothetical protein
General function prediction only [R]
CBO1449	COG2234		[R]	+4.6	Membrane protein
CBO0670	COG2984		[R]	−4.2	Lipoprotein
CBO2137	COG2234		[R]	+4.7	Membrane protein
Cell motility [N]
CBO2645	COG1298	flhA	[NU]	−7.9	Flagellar biosynthesis protein
CBO2652	COG1291	motA	[N]	−5.2	Chemotaxis MotA protein
Energy production and conversion [C]
CBO3577	COG0778		[C]	−5.2	Nitroreductase
Signal transduction mechanisms [T]
CBO0787	COG0642		[T]	+6.5	Two-component sensor kinase
CBO1120	COG0642		[T]	+4.3	Two-component sensor kinase
Cell cycle control, cell division, and chromosome partitioning [D]
CBO2535	COG0206	ftsZ	[D]	−4.7	Cell division protein FtsZ
Coenzyme transport and metabolism [H]
CBO2911	COG0422	thiC	[H]	−4.3	Thiamine biosynthesis protein
CBO2601	COG1057		[H]	+4.6	Transferase
Nucleotide transport and metabolism [F]
CBO0132	COG0504	pyrG	[F]	−4.7	CTP synthase
Carbohydrate transport and metabolism [G]
CBO1071	COG2271	glpT	[G]	+4.3	Glycerol-3-phosphate transporter
Secondary metabolites biosynthesis, transport, and catabolism [Q]
CBO1169	COG2313		[Q]	+4.3	Indigoidine synthase A family protein