Table 1: The top 40 differentially expressed genes in response to heat shock stress in Clostridium botulinum ATCC 3502.

Locus tagCOGGene nameFunction classFold changeFunction description

Posttranslational modification, protein turnover, and chaperones [O]
CBO2958COG0484dnaJ [O]+8.9Chaperone protein
CBO2959COG0443dnaK [O]+17.2Chaperone protein
CBO0189COG0484[O]+8.2DnaJ-related protein
CBO2960COG0576grpE [O]+5.6Heat shock protein
CBO3299COG0234groES [O]+14.210 kDa chaperonin GroES
CBO3298COG0459groEL [O]+8.360 kDa chaperonin GroEL
CBO0501COG2077tpx [O]+5.0Thiol peroxidase
CBO0803ha-33 +1.8Toxin cluster gene ha-33
CBO0802ha-17 +1.6Toxin cluster gene ha-17

Transcription [K]
CBO0645COG1846[K]+16.1MarR-family transcriptional regulator
CBO1012COG1846[K]+7.7MarR-family transcriptional regulator
CBO1017COG4978[KT]−5.8MerR-family transcriptional regulator
CBO2304COG1695[K]+5.8Transcriptional regulator, PadR family
CBO0786COG0745[KT]+4.4Two component response regulator
CBO0100COG1609ccpA [K]−7.3LacI-family transcriptional regulator
CBO2938COG0568rpoD [K]+4.2RNA polymerase sigma factor (sigma-43)
CBO3519COG0782greA [K]−5.0Transcription elongation factor

Translation and ribosomal structure and biogenesis [J]
CBO3164COG0525valS [J]−12.1Valyl-tRNA synthetase
CBO3069COG0809queA [J]−9.2tRNA ribosyltransferase-isomerase
CBO1204COG0162tyrR [J]−5.6Tyrosyl-tRNA synthetase
CBO3265COG0064gatB [J]−4.3Glutamyl-tRNA amidotransferase subunit B
CBO3503COG0442proS2 [J]−4.3Prolyl-tRNA synthetase
CBO3323COG0162tyrS [J]−4.7Tyrosyl-tRNA synthetase
CBO3470COG0198rplX [J]−6.750S ribosomal protein L24
CBO3474COG0092rpsC [J]−4.630S ribosomal protein S3
CBO0400COG0465ftsH [J]−6.3Cell division protein

Amino acid transport and metabolism [E]
CBO1675COG0747[E]+6.8Oligopeptide-binding protein
CBO1860COG0697ecsC [E]+4.1Membrane protein

Cell wall, membrane, and envelope biogenesis [M]
CBO2101COG1087[M]−4.2UDP-glucose epimerase
CBO3597COG0764fabZ [M]+8.3(3R)-Hydroxymyristoyl-ACP dehydratase

Hypothetical proteins
CBO3522−4.1Hypothetical protein
CBO2185+5.3Hypothetical protein
CBO2640−6.3Hypothetical protein
CBO2096+5.8Hypothetical protein
CBO3353+7.6Hypothetical protein

Function unknown [S]
CBO1070COG3862[S]+6.0Hypothetical protein
CBO0014COG3862[S]−4.2Hypothetical protein
CBO2935COG0327[S]+4.1Hypothetical protein

General function prediction only [R]
CBO1449COG2234[R]+4.6Membrane protein
CBO0670COG2984[R]−4.2Lipoprotein
CBO2137COG2234[R]+4.7Membrane protein

Cell motility [N]
CBO2645COG1298flhA [NU]−7.9Flagellar biosynthesis protein
CBO2652COG1291motA [N]−5.2Chemotaxis MotA protein

Energy production and conversion [C]
CBO3577COG0778[C]−5.2Nitroreductase

Signal transduction mechanisms [T]
CBO0787COG0642[T]+6.5Two-component sensor kinase
CBO1120COG0642[T]+4.3Two-component sensor kinase

Cell cycle control, cell division, and chromosome partitioning [D]
CBO2535COG0206ftsZ [D]−4.7Cell division protein FtsZ

Coenzyme transport and metabolism [H]
CBO2911COG0422thiC [H]−4.3Thiamine biosynthesis protein
CBO2601COG1057[H]+4.6Transferase

Nucleotide transport and metabolism [F]
CBO0132COG0504pyrG [F]−4.7CTP synthase

Carbohydrate transport and metabolism [G]
CBO1071COG2271glpT [G]+4.3Glycerol-3-phosphate transporter

Secondary metabolites biosynthesis, transport, and catabolism [Q]
CBO1169COG2313[Q]+4.3Indigoidine synthase A family protein