761451.fig.004a
(a)
761451.fig.004b
(b)
Figure 4: Induction of reactive oxygen species (ROS) within HaCaT cells by plasma. HaCaT cells remained either untreated (time 0) or were treated with DBD/air ((a);  h after plasma treatment, ○ = 24 h after plasma treatment; ) or DBD/argon ((b);  h after plasma treatment, ○ = 24 h after plasma treatment; ) plasma for 60 to 300 s. Intracellular ROS were detected by flow cytometry using the dye CM-H2DCFDA and results are given as mean ± SEM. , versus control cells ( -test).