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BioMed Research International
Volume 2013 (2013), Article ID 762132, 15 pages
Review Article

Engineering Parameters in Bioreactor’s Design: A Critical Aspect in Tissue Engineering

1Department of Chemical Engineering, Faculty of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, Iran
2Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, P.O. Box 14395-1374, Tehran, Iran
3Institute of Physiology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany
4National Cell Bank, Pasteur Institute of Iran, P.O. Box 1316943551, Tehran, Iran
5Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands

Received 9 April 2013; Revised 27 June 2013; Accepted 1 July 2013

Academic Editor: Kacey Gribbin Marra

Copyright © 2013 Nasim Salehi-Nik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Bioreactors are important inevitable part of any tissue engineering (TE) strategy as they aid the construction of three-dimensional functional tissues. Since the ultimate aim of a bioreactor is to create a biological product, the engineering parameters, for example, internal and external mass transfer, fluid velocity, shear stress, electrical current distribution, and so forth, are worth to be thoroughly investigated. The effects of such engineering parameters on biological cultures have been addressed in only a few preceding studies. Furthermore, it would be highly inefficient to determine the optimal engineering parameters by trial and error method. A solution is provided by emerging modeling and computational tools and by analyzing oxygen, carbon dioxide, and nutrient and metabolism waste material transports, which can simulate and predict the experimental results. Discovering the optimal engineering parameters is crucial not only to reduce the cost and time of experiments, but also to enhance efficacy and functionality of the tissue construct. This review intends to provide an inclusive package of the engineering parameters together with their calculation procedure in addition to the modeling techniques in TE bioreactors.