Engineering Parameters in Bioreactor’s Design: A Critical Aspect in Tissue Engineering

Figure 1

Comparison between unsteady state model and experimental results for the partial pressure of oxygen in the headspace of the ventilation flasks f1, f4, f7, f9 (sterile plug dimensions in f1<f4<f7<f9) is obtained for the fermentation of C. glutamicum DM 1730 on 10 g/L glucose and 21 g/L MOPS (𝑉_𝐿=10 mL, 𝑛=400 rpm, 𝑇=30^∘C, 𝑑_𝑜=5 cm, 𝑌_𝑥/𝑠=0.48, 𝑌_𝑥/𝑜2=53 g/mol, RQ=1 where 𝑑_𝑜, 𝑉_𝐿, 𝑌_𝑥/𝑠, 𝑌_𝑥/𝑜2, and RQ are shaking diameter, filling volume, yield of biomass with respect to substrate, yield of biomass with respect to oxygen, and respiration quotient, resp.).