Moscatilin Inhibits Lung Cancer Cell Motility and Invasion via Suppression of Endogenous Reactive Oxygen Species
Figure 4
Effects of moscatilin on H23 cell invasion. (a) H23 cells were treated with various nontoxic doses of moscatilin (0-1 μM) for 24 h. (b) H23 cells were treated with moscatilin (1 μM) or left untreated as control for various times (12–48 h). Cell invasion was evaluated using transwell coated with matrigel as described under Section 2. Invaded cells across the membrane were stained with Hoechst33342 for 30 min. Data were plotted as an average number of cells in each field and represented the means ± SD (). versus nontreated control cells. (c) Invading cells were stained with Hoechst33342 and visualized under fluorescence microscopy. (d) Effect of moscatilin on filopodia formation and cell morphology. After being treated with nontoxic dose of moscatilin for 24 h, cells were stained with either phalloidin or sulforhodamine B and visualized under fluorescence microscope. Filopodia was indicated by arrow.
