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Figure 4: An outline figure of 2D-DIGE. Proteins are extracted from the samples and are labelled with different fluorophores as Cy 3 for sample 1, Cy 5 for sample 2, and Cy 2 for the pooled internal standard. All the samples are resolved in the same 2D gel followed by protein spot pattern detection by scanning the gel in respective wavelength for the Cy dyes; the merging of all of them yields an overlay image consisting of all three Cy dyes. The images are analyzed to get potential candidates of interest.