Molecular Characterization and In Silico Analysis of Naturally Occurring TEM Beta-Lactamase Variants among Pathogenic Enterobacteriaceae Infecting Indian Patients

Table 3

Bla _TEM protein stability analysis at five differentiating amino acid positions.

Amino acid substitution	Change in solvent; accessibility (%)	Free folding energy change (ΔΔG) (kcal/mol)
Met69Leu	1	2.0
Leu69Met	0.55	−3.82
Val84Ile	24.72	−0.48
Ile84Val	15.14	−1.86
Trp165Gly	37.84	0.9
Gly165Trp	34.31	−1.61
Ala184Val	27.22	0.59
Val184Ala	15.61	−1.25
Asn276Asp	8.2	0.09
Asp276Asn	9.72	−1.59