Cytotoxic Effect of Ethanol Extract of Microalga, Chaetoceros calcitrans, and Its Mechanisms in Inducing Apoptosis in Human Breast Cancer Cell Line
Table 1
Selected apoptotic and cell cycle related genes used in GeXP assay.
Gene
Accession number
Product size
Left sequence*
Right Sequence**
BCL2
M14745
157
ACCACTAATTGCCAAGCACC
TTTTCCATCCGTCTGCTCTT
Fas
NM_000043
165
CTCCAAGGGATTGGAATTGA
TGCAGTCCCTAGCTTTCCTT
TNF alpha
NM_000594
171
CTATCTGGGAGGGGTCTTCC
ATGTTCGTCCTCCTCACAGG
Caspase 3
NM_004346
182
GAACTGGACTGTGGCATTGA
ACCAGGAGCCATCCTTTGA
p21Cip1
NM_000389
202
TGTGGACCTGTCACTGTCTTG
TAGGGCTTCCTCTTGGAGAA
Cyclin A2
NM_001237
212
TATTGCTGGAGCTGCCTTTC
CTTTTCTCTTATTGACTGTTGTGCAT
BCL2L1
NM_001191
232
CCACAGCAGCAGTTTGGAT
GGGATTGTTCCCATAGAGTTCCACAA
MDM2
NM_002392
239
GGTGGGAGTGATCAAAAGGA
ACCAGGCTTTCATCAAAGGAA
Caspase 7
NM_033340
247
CAGACCGGTCCTCGTTTGTA
ACCTCGGCATCTTTGTCTGTT
CDK2
NM_052827
285
TGGTGGCGCTTAAGAAAATC
ACAGCTGGAACAGATAGCTCTTGA
ACTBa
NM_001101
295
CTGGCACCACACCTTCTACA
AAGGGCATACCCCTCGTAGAT
Bax
BC014175
316
CCCTTTTGCTTCAGGGTTTC
ACAAAGTAGAAAAGGGCGACAA
KANb
Kan(r)
325
ATCATCAGCATTGCATTCGATTCCTGTTTG
AATTCCGACTCGTCCAACATC
Caspase 9
NM_001229
332
GGGCTCACTCTGAAGACCTG
ATCTGGAAGCTGCTAAGAGCC
P53
NM_000546
340
TTTTGGGTTTTGGGTCTTTG
ATTCAACATGAGGGACAGCTT
*Forward universal primer sequence (AGGTGACACTATAGAATA).
**Reverse universal primer sequence (GTACGACTCACTATAGGGA).
aGene used for normalization.
bInternal control.
