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BioMed Research International
Volume 2013 (2013), Article ID 783809, 6 pages
http://dx.doi.org/10.1155/2013/783809
Research Article

The Effect of Thiamine and Thiamine Pyrophosphate on Oxidative Liver Damage Induced in Rats with Cisplatin

1Department of Pediatrics, Faculty of Medicine, Ataturk University, Erzurum 25240, Turkey
2Department of Internal Medicine, Pasinler State Hospital, Erzurum 25300, Turkey
3Department of Pharmacology, Ataturk University, Erzurum 25240, Turkey
4Department of Biochemistry, Erzurum Research and Educational Hospital, Erzurum 25070, Turkey
5Department of Surgery, Ataturk University, Erzurum 25240, Turkey

Received 3 April 2013; Revised 21 May 2013; Accepted 23 May 2013

Academic Editor: Elvira Gonzalez De Mejia

Copyright © 2013 Mehmet Ibrahim Turan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the effect of thiamine and thiamine pyrophosphate (TPP) on oxidative stress induced with cisplatin in liver tissue. Rats were divided into four groups; thiamine group (TG), TPP + cisplatin group (TPG), healthy animal group (HG), and cisplatin only group (CG). Oxidant and antioxidant parameters in liver tissue and AST, ALT, and LDH levels in rat sera were measured in all groups. Malondialdehyde levels in the CG, TG, TPG, and HG groups were 11 ± 1.4, 9 ± 0.5, 3 ± 0.5, and 2.2 ± 0.48 μmol/g protein, respectively. Total glutathione levels were 2 ± 0.7, 2.8 ± 0.4, 7 ± 0.8, and 9 ± 0.6 nmol/g protein, respectively. Levels of 8-OH/Gua, a product of DNA damage, were 2.7 ± 0.4 pmol/L, 2.5 ± 0.5, 1.1 ± 0.3, and 0.9 ± 0.3 pmol/L, respectively. A statistically significant difference was determined in oxidant/antioxidant parameters and AST, ALT, and LDH levels between the TPG and CG groups ( ). No significant difference was determined between the TG and CG groups ( ). In conclusion, cisplatin causes oxidative damage in liver tissue. TPP seems to have a preventive effect on oxidative stress in the liver caused by cisplatin.