α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli
Figure 1
In situ ICDH and GDH activity and transcriptional level of gdhA and icdA genes in tellurite-exposed E. coli. ICDH (a) and (b) GDH activity was assayed after fractionating extracts from tellurite-treated cells by native gradient polyacrylamide gels as described in methods. Representative gels are shown. (c) Transcriptional level of gdhA and icdA genes in tellurite-exposed E. coli. Numbers represent the mean of 3 independent trials. The relative expression was normalized to that of the rpoD gene.