α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli

Figure 2

Isocitrate and α-KG content in tellurite-exposed E. coli. (a), Isocitrate content was assessed by HPLC in extracts from wild type E. coli previously exposed to 0.5 μg/mL tellurite for 5, 15 and 30 min as described in Methods. Numbers represent the mean of 3 independent trials ± SD. ^∗𝑃<0.005. (b), α-KG content in E. coli previously grown in LB medium. α-KG content was determined by HPLC in cell-free extracts of the indicated E. coli strains as described in Methods. Bars represent the standard deviation (𝑛=3). ^∗𝑃<0.005.

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