α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli

Table 1

E.  coli strains and primers.

Strain	Relevant genotype	Reference
BW25113	lacIq rrnB ΔlacZ hsdR514 ΔaraBAD ΔrhaBAD	[11]
ΔgdhA	BW25113 ΔgdhA (gdhA::kan)	[11]
ΔicdA	BW25113 ΔicdA (icdA::kan)	[11]
ΔsodAB	DE(lac)4169 rpsL DE(sodA-lacZ)49; DE(sodB-kan)1-DE(2) Cam^R Kan^R	[11]
icdApCA24N	BW25113 carrying pCA24N	[11]
Primers	Forward (F) or reverse (R), to amplify	5′-3′ sequence
gdhA F	F, gdhA	CATATTCTCTGGAGTCATTCCTCA
gdhA R	R, gdhA	ATCATCAACCCA TACCACGC
icdA F	F, icdA	TCCGGCACAAGGCAAGAAGA
icdA R	R, icdA	CAGCCAGACGTCCTGACCAT