Figure 5: Effect of pMTX-II, pMTX-III, and pMTX-IV on phagocytosis (a) and production (b) by J774A.1 macrophages. The phagocytosis of nonopsonized zymosan particles was determined by phase-contrast microscopy. J774A.1 macrophages were incubated with 6 μg/mL of pMTX-II, pMTX-III,pMTX-IV, and RPMI (control) during 60 minutes before addition of non-opsonized zymosan particles. Values represent the mean ± S.D.M. from 3–5 independent experiments. * compared with control # compared with positive control (ANOVA).