Late Adherent Human Bone Marrow Stromal Cells Form Bone and Restore the Hematopoietic Microenvironment In Vivo

Figure 9

Implants loaded with L-cells. Note that the hematopoietic microenvironment is reconstituted in vivo. (a) Interconnecting bone formation is seen on the surface of HA/TCP particles. A small cluster of hematopoietic tissue is in close association with a blood vessel. (b) Well-organized bone deposition reproduces the classical microanatomy of cancellous bone. The hematopoietic tissue clearly shows a spatial relationship with the blood vessels and with bone. (c) Detail from the hematopoietic tissue containing cells of the erythroid (lower left), myelocytic (mi) and megakaryocytic (me) series in close association with the vascular network. Structural maturity of the bone is verified by the orderly disposition of osteocytes within the bone. Four micra paraffin-embedded sections stained by H&E. Scale bars = 100 μm.