Table 3: Running times in minutes for mapping NGS reads to a reference genome using Bowtie based on the use of traditional computer cluster. The column titled upload_speed specifies the upload speed. The column titled “upload” includes the time in minutes for uploading the data to the cloud with the respective upload speed. The column titled “compTime” includes the computation time in minutes of the whole dataset after being uploaded to the cloud. The column titled totalTimeS includes the experiment time in streaming mode and the column titled totalTimeT includes the time in nonstreaming mode, where all the data is first transferred and then processed. The numbers in brackets in this column are the respective monetary cost.

Upload_speed Read size Nodes Upload CompTime TotalTimeT TotalTimeS

E. coli reads

250 KB/s 1.4 G 1 100 3 103 ($1.32) 102 ($1.32)
250 KB/s 1.4 G 2 100 3 103 ($1.98) 102 ($2.64 )
250 KB/s 1.4 G 4 100 3 103 ($3.3) 102 ($5.28)

1 GB human reads

250 KB/s 1 G 1 71 17 88 ($1.32) 72 ($1.32)
250 KB/s 1 G 2 71 11 82 ($1.98) 72 ($2.64)
250 KB/s 1 G 4 71 7 73 ($3.3) 72 ($5.28)

10 GB human reads

250 KB/s 10 G 1 800 (13.3 h) 220 1021 ($11.88) 832 ($9.24)
250 KB/s 10 G 2 800 (13.3 h) 130 930 ($12.54) 818 ($9.24)
250 KB/s 10 G 4 800 (13.3 h) 60 860 ($11.88) 818 ($9.24)

E. coli reads

1 MB/s 1.4 G 1 25 3 28 ($0.66) 27 ($0.66)
1 MB/s 1.4 G 2 25 3 28 ($1.32) 27 ($1.32)
1 MB/s 1.4 G 4 25 3 28 ($2.64) 27 ($2.64)

1 GB human reads

1 MB/s 1 G 1 18 17 35 ($0.66) 21 ($0.66)
1 MB/s 1 G 2 18 11 29 ($1.32) 21 ($1.32)
1 MB/s 1 G 4 18 7 25 ($2.64) 21 ($2.64)

10 GB human reads

1 MB/s 10 G 1 200 220 421 ($5.28) 231 ($2.64)
1 MB/s 10 G 2 200 130 330 ($5.94) 215 ($5.28)
1 MB/s 10 G 4 200 60 261 ($5.28) 215 ($10.56)

40 GB human reads

1 MB/s 40 G 1 690 590 1280 ($14.52) 1100 ($12.54)
1 MB/s 40 G 2 690 325 1015 ($15.18) 695 ($15.84)
1 MB/s 40 G 4 690 180 870 ($15.84) 695 ($31.68)

130 GB human reads

1 MB/s 130 G 1 2220 1720 3940 ($43.56) 3600 ($39.6)
1 MB/s 130 G 2 2220 940 3160 ($45.54) 2400 ($52.8)
1 MB/s 130 G 4 2220 520 2740 ($48.18) 2400 ($105.6)
1 MB/s 130 G 8 2220 284 2504 ($50.82) 2400 ($211.2)

E. coli reads

10 MB/s 1.4 G 1 2.5 3 5.5 ($0.66) 5 ($0.66)
10 MB/s 1.4 G 2 2.5 3 5.5 ($1.32) 5 ($1.32)
10 MB/s 1.4 G 4 2.5 3 5.5 ($2.64) 5 ($2.64)

10 GB human reads

10 MB/s 10 G 1 18 220 238 ($2.64) 180 ($1.98)
10 MB/s 10 G 2 18 130 148 ($3.96) 85 ($2.64)
10 MB/s 10 G 4 18 60 78 ($3.3) 50 ($2.64)

40 GB human reads

10 MB/s 40 G 1 70 590 660 ($7.26) 686 ($7.92)
10 MB/s 40 G 2 70 310 380 ($8.58) 350 ($7.92)
10 MB/s 40 G 4 70 170 240 ($8.58) 180 ($7.92)
10 MB/s 40 G 8 70 95 165 ($11.22) 100 ($10.56)
10 MB/s 40 G 16 70 53 123 ($11.88) 73 ($21.12)

130 GB human reads

10 MB/s 130 G 1 224 1720 1944 ($21.78) 2050 ($23.1)
10 MB/s 130 G 2 224 950 1174 ($23.76) 1100 ($25.08)
10 MB/s 130 G 4 224 520 744 ($26.4) 580 ($26.4)
10 MB/s 130 G 8 224 284 508 ($33.66) 320 ($31.68)
10 MB/s 130 G 16 224 160 384 ($34.32) 235 ($42.24)