Table 4: Running times in minutes for mapping NGS reads to a reference genome using Crossbow. The column titled speed specifies the upload speed. The column titled cluster includes the number of created EMR clusters. The column titled compTime incudes the computation time of the whole data after uploading it. The column titled totalTimeS includes the experiment time in streaming mode, and the column titled totalTimeT includes the time in nonstreaming mode, where all the data is first transferred and then processed. The number in brackets in this column is the respective monetary cost.

Upload_speed Read_size Clusters Upload_time CompTime TotalTimeT TotalTimeS

E. coli reads

250 KB/s 1.4 G 1 100 9 109 ($0.72) 110 ($0.96)
250 KB/s 1.4 G 2 100 7 107 ($1.2) 110 ($1.92)
250 KB/s 1.4 G 4 100 7 107 ($2.26) 110 ($3.84)

1 GB human reads

250 KB/s 1 G 1 71 6 77 ($0.72) 84 ($0.96)
250 KB/s 1 G 2 71 5 76 ($1.2) 84 ($1.92)
250 KB/s 1 G 4 71 5 76 ($1.2) 84 ($3.84)

10 GB human reads

250 KB/s 10 G 1 800 60 860 ($2.16) 820 ($6.82)
250 KB/s 10 G 2 800 31 831 ($2.64) 820 ($13.44)
250 KB/s 10 G 4 800 18 818 ($3.6) 820 ($26.88)

E. coli reads

1 MB/s 1.4 G 1 25 9 34 ($0.6) 65 ($0.96)
1 MB/s 1.4 G 2 25 7 32 ($1.08) 65 ($1.92)
1 MB/s 1.4 G 4 25 7 32 ($2.04) 65 ($3.84)

1 GB human reads

1 MB/s 1 G 1 18 6 24 ($0.6) 31 ($0.48)
1 MB/s 1 G 2 18 5 23 ($1.09) 32 ($0.96)
1 MB/s 1 G 4 18 5 23 ($2.04) 31 ($0.1.92)

10 GB human reads

1 MB/s 10 G 1 200 60 260 ($0.96) 220 ($1.92)
1 MB/s 10 G 2 200 31 231 ($1.54) 220 ($3.84)
1 MB/s 10 G 4 200 18 218 ($2.40) 220 ($7.68)

40 GB human reads

1 MB/s 40 G 1 690 580 1270 ($6.24) 630 ($5.28)
1 MB/s 40 G 2 690 300 990 ($6.24) 630 ($10.56)
1 MB/s 40 G 4 690 150 840 ($7.2) 630 ($21.12)
1 MB/s 40 G 8 690 80 770 ($9.12) 630 ($42.24)

130 GB human reads

1 MB/s 130 G 1 2220 1890 4110 ($19.8) 2360 ($19.2)
1 MB/s 130 G 2 2220 945 3165 ($19.8) 2320 ($37.44)
1 MB/s 130 G 4 2220 476 2696 ($19.8) 2320 ($74.88)
1 MB/s 130 G 8 2220 184 2404 ($19.8) 2320 ($149.76)
1 MB/s 130 G 16 2220 126 2346 ($27.48) 2320 ($299.52)

10 GB human reads

10 MB/s 10 G 1 20 60 80 ($1.08) 160 ($1.44)
10 MB/s 10 G 2 20 31 51 ($1.08) 95 ($1.92)
10 MB/s 10 G 4 20 18 38 ($2.04) 65 ($3.84)

40 GB human reads

10 MB/s 40 G 1 70 580 650 ($5.04) 610 ($5.28)
10 MB/s 40 G 2 70 300 370 ($5.04) 310 ($5.76)
10 MB/s 40 G 4 70 150 220 ($6.00) 170 ($5.76)
10 MB/s 40 G 8 70 80 150 ($7.92) 110 ($7.68)

130 GB human reads

10 MB/s 130 G 1 224 1890 2114 ($15.84) 1960 ($15.84)
10 MB/s 130 G 2 224 945 1169 ($15.84) 1000 ($16.32)
10 MB/s 130 G 4 224 476 700 ($15.84) 520 ($17.28)
10 MB/s 130 G 8 224 184 470 ($15.84) 300 ($19.2)
10 MB/s 130 G 16 224 126 350 ($23.52) 300 ($38.4)