Mechanisms by Which Interleukin-6 Attenuates Cell Invasion and Tumorigenesis in Human Bladder Carcinoma Cells
Figure 3
Expression of IL6 modulates NDRG1, MASPIN, and KAI1 gene expression in bladder carcinoma cells. (a) The expression profiles of the NDRG1, MASPIN, and KAI1 proteins in IL6-transfected cells (HT-IL6) and mock-transfected control cells (HT-DNA) were determined by immunoblotting analysis. (c) The expression profiles of the NDRG1 and MASPIN proteins in IL6-knockdown T24 cells and mock-IL6-knockdown T24 cells (T24-GFPsi) were determined by immunoblotting analysis. Quantitative analysis was based on the intensity of the protein bands produced by the expression of the target genes and the β-actin internal control gene in 3 independent experiments (b, d). The fold-induction data are presented as the band intensity corresponding to the target gene/β-actin gene (±SE) relative to the corresponding band intensity for the mock-treated cells. (e) The NDRG1 (black bars), MASPIN (white bars), and the KAI1 (gray bars) reporter vector-transfected HT1376 cells were cotransfected with different concentrations of IL6 expression vector for 72 h. (f) The NDRG1 (black bars), MASPIN (white bars), and the KAI1 (gray bars) reporter vector-transfected HT1376 cells were treated with different concentrations of exogenous recombinant human IL6 for 48 h. Cells were lysed for the luciferase assays. Data are presented as mean percentage ±SE. () relative to the control groups. *.
