791212.fig.004a
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791212.fig.004b
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791212.fig.004c
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Figure 4: IL6 modulates the expression of E-cadherin, N-cadherin, and vimentin in bladder carcinoma cells. (a) The different levels of expression of E-cadherin, N-cadherin, and vimentin between HT-DNA and HT-IL6 cells (left) and between T24-GFPsi and T24-IL6si cells (right) were determined by immunoblotting analysis. (b) The quantitative analysis of HT-IL6/HT-DNA cells (black bars) and T24-IL6si/T24-GFPsi cells (white bars) was based on the intensity of the protein bands produced by the expression of the target genes and the β-actin internal control gene in 3 independent experiments. The fold-induction data are expressed as of the intensity of the protein bands produced from the target gene/β-actin (±SE) relative to that of the mock-treated cells. (c) The different levels of expression of vimentin between HT-DNA and HT-IL6 cells (left) and between T24-GFPsi and T24-IL6si cells (right) were determined by RT-PCR analysis.