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BioMed Research International
Volume 2013 (2013), Article ID 793502, 8 pages
http://dx.doi.org/10.1155/2013/793502
Research Article

Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry

1Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, Bethesda, MD 20892, USA
2Department of Pathology and Laboratory Medicine, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA 90073, USA

Received 30 November 2012; Accepted 28 January 2013

Academic Editor: Akanchha Kesari

Copyright © 2013 Gouri Shankar Pandey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. O. D. Ratnoff and B. Bennett, “The genetics of hereditary disorders of blood coagulation,” Science, vol. 179, no. 4080, pp. 1291–1298, 1973. View at Scopus
  2. J. Graw, H. H. Brackmann, J. Oldenburg, R. Schneppenheim, M. Spannagl, and R. Schwaab, “Haemophilia A: from mutation analysis to new therapies,” Nature Reviews Genetics, vol. 6, no. 6, pp. 488–501, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. P. M. Mannucci and E. G. D. Tuddenham, “The hemophilias—from royal genes to gene therapy,” New England Journal of Medicine, vol. 344, no. 23, pp. 1773–1779, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. D. Lillicrap, “Improvements in factor concentrates,” Current Opinion in Hematology, vol. 17, no. 5, pp. 393–397, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Wight and S. Paisley, “The epidemiology of inhibitors in haemophilia A: a systematic review,” Haemophilia, vol. 9, no. 4, pp. 418–435, 2003. View at Scopus
  6. B. Levinson, S. Kenwrick, D. Lakich, G. Hammonds Jr., and J. Gitschier, “A transcribed gene in an intron of the human factor VIII gene,” Genomics, vol. 7, no. 1, pp. 1–11, 1990. View at Publisher · View at Google Scholar · View at Scopus
  7. S. E. Antonarakis, J. P. Rossiter, M. Young et al., “Factor VIII gene inversions in severe hemophilia A: results of an international consortium study,” Blood, vol. 86, no. 6, pp. 2206–2212, 1995. View at Scopus
  8. A. H. Zhang, J. Skupsky, and D. W. Scott, “Factor VIII inhibitors: risk factors and methods for prevention and immune modulation,” Clinical Reviews in Allergy and Immunology, vol. 37, no. 2, pp. 114–124, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. K. R. Viel, A. Ameri, T. C. Abshire, et al., “Inhibitors of factor VIII in black patients with hemophilia,” New England Journal of Medicine, vol. 360, no. 16, pp. 1618–1627, 2009. View at Publisher · View at Google Scholar
  10. M. Young, H. Inaba, L. W. Hoyer, M. Higuchi, H. H. Kazazian, and S. E. Antonarakis, “Partial correction of a severe molecular defect in hemophilia A, because of errors during expression of the factor VIII gene,” American Journal of Human Genetics, vol. 60, no. 3, pp. 565–573, 1997. View at Scopus
  11. S. C. Gouw, H. M. Berg, J. Oldenburg, et al., “F8 gene mutation type and inhibitor development in patients with severe hemophilia A: systematic review and meta-analysis,” Blood, vol. 119, no. 12, pp. 2922–2934, 2012. View at Publisher · View at Google Scholar
  12. D. Greenbaum, C. Colangelo, K. Williams, and M. Gerstein, “Comparing protein abundance and mRNA expression levels on a genomic scale,” Genome Biology, vol. 4, no. 9, article 117, 2003. View at Publisher · View at Google Scholar · View at Scopus
  13. J. W. Parker, “Flow cytometry in the diagnosis in hematologic diseases,” Annals of Clinical and Laboratory Science, vol. 16, no. 6, pp. 427–442, 1986. View at Scopus
  14. R. L. Johnson, “Flow cytometry: from research to clinical laboratory applications,” Clinics in Laboratory Medicine, vol. 13, no. 4, pp. 831–852, 1993. View at Scopus
  15. K. Mous, W. Jennes, A. de Roo, I. Pintelon, L. Kestens, and X. van Ostade, “Intracellular detection of differential APOBEC3G, TRIM5alpha, and LEDGF/p75 protein expression in peripheral blood by flow cytometry,” Journal of Immunological Methods, vol. 372, no. 1-2, pp. 52–64, 2011. View at Publisher · View at Google Scholar
  16. Z. E. Sauna, C. Okunji, R. C. Hunt et al., “Characterization of conformation-sensitive antibodies to ADAMTS13, the von Willebrand cleavage protease,” PLoS One, vol. 4, no. 8, Article ID e6506, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. R. J. Summers, S. L. Meeks, J. F. Healey et al., “Factor VIII A3 domain substitution N1922S results in hemophilia A due to domain-specific misfolding and hyposecretion of functional protein,” Blood, vol. 117, no. 11, pp. 3190–3198, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Egler, T. Albert, O. Brokemper et al., “Kinetic parameters of monoclonal antibodies ESH2, ESH4, ESH5, and ESH8 on coagulation factor VIII and their influence on factor VIII activity,” Journal of Molecular Recognition, vol. 22, no. 4, pp. 301–306, 2009. View at Publisher · View at Google Scholar · View at Scopus
  19. B. D. Griffin, L. R. Micklem, and M. C. McCann, “The production and characterisation of a panel of ten murine monoclonal antibodies to human procoagulant Factor VIII,” Thrombosis and Haemostasis, vol. 55, no. 1, pp. 40–46, 1986. View at Scopus
  20. D. Scandella, G. E. Gilbert, M. Shima et al., “Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248 through 2312, which overlap a phospholipid-binding site,” Blood, vol. 86, no. 5, pp. 1811–1819, 1995. View at Scopus
  21. X. Li, G. Jiang, D. Wu, X. Wang, and B. Zeng, “Construction of a recombinant eukaryotic expression plasmid containing human calcitonin gene and its expression in NIH3T3 cells,” Journal of Biomedicine and Biotechnology, vol. 2009, Article ID 241390, 7 pages, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. Baxter, Advate Prescribing Information, Baxter, Deerfield, Mass, USA, 2010.
  23. Bayer HealthCare, Kogenate Prescribing Information, Bayer HealthCare, Leverkusen, Germany, 2009.
  24. Wyeth Pharma (Pfizer), ReFacto Prescribing Information, Wyeth Pharma (Pfizer), Madison, Wis, USA, 2007.
  25. X. Guo, H. Wang, H. Chu et al., “[Retroviral-mediated high efficient in vitro expression of human coagulation factor VIII],” Zhonghua xue ye xue za zhi, vol. 21, no. 9, pp. 457–459, 2000. View at Scopus
  26. K. Swiech, A. Kamen, S. Ansorge, et al., “Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII,” BMC Biotechnology, vol. 11, article 114, 2011.