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BioMed Research International
Volume 2013 (2013), Article ID 794643, 10 pages
http://dx.doi.org/10.1155/2013/794643
Research Article

MLST Genotypes and Antibiotic Resistance of Campylobacter spp. Isolated from Poultry in Grenada

1School of Veterinary Medicine, St. George's University, P.O. Box 7, Grenada, West Indies
2College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA

Received 7 November 2012; Revised 28 December 2012; Accepted 7 January 2013

Academic Editor: Fabio Ribeiro Braga

Copyright © 2013 Diana Stone et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study determined whether multilocus sequence types (MLST) of Campylobacter from poultry in 2 farms in Grenada, West Indies, differed by farm, antimicrobial resistance and farm antibiotic use. Farm A used fluoroquinolones in the water and Farm B used tetracyclines. The E-test was used to determine resistance of isolates to seven antibiotics. PCR of the IpxA gene confirmed species and MLST was used to characterize 38 isolates. All isolates were either C. jejuni or C. coli. Farm antibiotic use directly correlated with antimicrobial resistance of Campylobacter isolates. Almost 80% of the isolates from Farm A were fluoroquinolone resistant and 17.9% of the isolates from Farm B were fluoroquinolone resistant. All Campylobacter isolates from Farm A were tetracycline sensitive, whereas 35.7% of isolates from Farm B were tetracycline resistant. Six previously recognized sequence types (STs) and 2 novel STs were identified. Previously recognized STs were those overwhelmingly reported from poultry and humans globally. Isolates with the same ST did not always have the same antibiotic resistance profile. There was little ST overlap between the farms suggesting that within-farm transmission of Campylobacter genotypes may dominate. MLST typing was useful for tracking Campylobacter spp. among poultry units and can help elucidate Campylobacter host-species population structure and its relevance to human health.