Low-Cytotoxic Synthetic Bromorutaecarpine Exhibits Anti-Inflammation and Activation of Transient Receptor Potential Vanilloid Type 1 Activities

Figure 1

Effects of bromo-dimethoxyrutaecarpine (Br-RUT) on nitric oxide (NO) and tumor necrosis factor (TNF)-α releases by lipopolysaccharide (LPS)-treated (100 ng/mL) RAW 264.7 macrophages. (a) NO levels were detected in culture medium using the Griess reaction. The percentage of untreated cells was set as the control to 100%. (b) TNF-α release in cell supernatants was detected using a mouse TNF-α Quantikine kit. (c) Cell viability upon Br-BUT treatment for 24 h in an MTT assay. Statistical significance is indicated compared to LPS treatment. (^∗𝑃<0.05, ^∗∗𝑃<0.001, ^∗∗∗𝑃<0.001).

(a)

(b)

(c)