Table 1: Comparison of expression levels of TLR signaling pathway and innate immunity genes in SIV-infected REG and RAP CD11b-depleted splenic cells. Expression levels were normalized using the average of 5 housekeeping genes and the ratio between RAP and REG animals’ dCT values was calculated issuing fold change. Shown are the genes whose expression is significantly changed between the groups, organized by functional categories of the TLRs and their adapter as well as interacting proteins, the downstream NFκB and JNK/p38 signaling pathways, as well as other related molecules (MAPK8IP3 is present in both the adapter/interacting and the JNK/p38 categories).

TLRs and adapter/interactingFold RAP/REG valueNFκB & JNK/p38Fold RAP/REG valueOtherFold RAP/REG value

GPC19.430.0168ESCIT10.050.0235CCL213.420.0193
HRAS5.720.0061IL1012.730.0497CD868.470.0216
LY869.960.0455IRAK28.070.0121CSF312.030.0161
MAL10.280.0028IRAK411.150.0310IL611.170.0239
MAPK8IP312.260.0031IRF39.740.0077IRF19.230.0006
MYD8812.390.0450JUN11.350.0387IRF714.370.0412
PGLYRP312.080.0388MAP2K311.650.0229LTA10.120.0196
SARM17.380.0335MAP2K48.800.0282PRKRA12.030.0258
TIRAP7.530.0275MAPK1210.050.0077TBK110.790.0476
TLR313.900.0142MAPK8IP312.260.0031
TLR64.100.0472NFKB28.700.0317
TLR95.600.0489NFKBIB12.670.0242
TOLLIP4.700.0082REL6.450.0270
RELB9.450.0401
TNF9.510.0034
TNFRSF1A8.120.0050