Table 2: Comparison of expression levels of TLR signaling pathway and innate immunity genes in SIV-infected REG and RAP CD8+ cells isolated from the brain. Expression levels were normalized using the average of 5 housekeeping genes and the ratio between RAP and REG animals’ dCT values was calculated issuing fold change. Shown are the genes whose expression is significantly changed between the groups, organized by functional categories of the TLRs and their adapter as well as interacting proteins, the downstream NFκB and JNK/p38 signaling pathways, as well as other related molecules.

TLRs and adapter/interactingFold RAP/REG valueNFκB & JNK/p38Fold RAP/REG valueOtherFold RAP/REG value

HRAS1.430.0436IL102.560.0373CCL227.490.0338
MYD882.550.0183IRF30.260.0123IL12B0.170.0387
PELI20.410.0011MAP2K31.620.0400IL23.380.0075
TLR11.950.0437MAP4K40.460.0040IRF13.080.0016
TLR24.600.0429MAPK101.380.0374IRF74.910.0009
TLR31.110.0417NFKB11.650.0151LTA1.270.0245
TLR40.510.0483NFKBIB2.030.0300PTGS22.570.0371
TLR82.640.0238NFKBIE1.370.0496TBK11.200.0276
TLR91.710.0242PPARA0.440.0489
TOLLIP1.780.0376REL1.880.0032
TNF4.470.0482
TNFRSF1A2.720.0090