Increased Toll-Like Receptor Signaling Pathways Characterize CD8+ Cells in Rapidly Progressive SIV Infection

Table 2

Comparison of expression levels of TLR signaling pathway and innate immunity genes in SIV-infected REG and RAP CD8+ cells isolated from the brain. Expression levels were normalized using the average of 5 housekeeping genes and the ratio between RAP and REG animals’ dCT values was calculated issuing fold change. Shown are the genes whose expression is significantly changed between the groups, organized by functional categories of the TLRs and their adapter as well as interacting proteins, the downstream NFκB and JNK/p38 signaling pathways, as well as other related molecules.

TLRs and adapter/interacting	Fold RAP/REG	𝑃 value	NFκB & JNK/p38	Fold RAP/REG	𝑃 value	Other	Fold RAP/REG	𝑃 value
HRAS	1.43	0.0436	IL10	2.56	0.0373	CCL2	27.49	0.0338
MYD88	2.55	0.0183	IRF3	0.26	0.0123	IL12B	0.17	0.0387
PELI2	0.41	0.0011	MAP2K3	1.62	0.0400	IL2	3.38	0.0075
TLR1	1.95	0.0437	MAP4K4	0.46	0.0040	IRF1	3.08	0.0016
TLR2	4.60	0.0429	MAPK10	1.38	0.0374	IRF7	4.91	0.0009
TLR3	1.11	0.0417	NFKB1	1.65	0.0151	LTA	1.27	0.0245
TLR4	0.51	0.0483	NFKBIB	2.03	0.0300	PTGS2	2.57	0.0371
TLR8	2.64	0.0238	NFKBIE	1.37	0.0496	TBK1	1.20	0.0276
TLR9	1.71	0.0242	PPARA	0.44	0.0489
TOLLIP	1.78	0.0376	REL	1.88	0.0032
			TNF	4.47	0.0482
			TNFRSF1A	2.72	0.0090