MHC Universal Cells Survive in an Allogeneic Environment after Incompatible Transplantation
Figure 2
Syngeneic and allogeneic transplantation models of stably MHC class I-silenced cells. (a) Fibrosarcoma cells expressing RT1-A molecules and control shRNA (shNS) or RT1-A-silenced fibrosarcoma cells derived from LEWis (LEW) rats after delivery of β2-microglobulin-specific shRNA (RN_shβ2m) were injected subcutaneously into the left hind leg of LEW rats (haplotype-matched setting) or LEW.1W rats (haplotype-mismatched setting). Histograms depict β2-microglobulin levels measured by real-time PCR (b) and MHC class I expression represented as mean fluorescence intensity (MFI) (c) on the fibroblast cells before the delivery of the shRNAs, immediately before transplantation, and two and eight weeks after transplantation.
