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BioMed Research International
Volume 2013 (2013), Article ID 796362, 15 pages
http://dx.doi.org/10.1155/2013/796362
Research Article

Identifying Cell Class Specific Losses from Serially Generated Electroretinogram Components

Department of Optometry and Vision Sciences, The University of Melbourne, Parkville, VIC 3010, Australia

Received 30 April 2013; Accepted 8 July 2013

Academic Editor: Mitsuru Nakazawa

Copyright © 2013 Christine T. O. Nguyen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Processing of information through the cellular layers of the retina occurs in a serial manner. In the electroretinogram (ERG), this complicates interpretation of inner retinal changes as dysfunction may arise from “upstream” neurons or may indicate a direct loss to that neural generator. We propose an approach that addresses this issue by defining ERG gain relationships. Methods. Regression analyses between two serial ERG parameters in a control cohort of rats are used to define gain relationships. These gains are then applied to two models of retinal disease. Results. The to gain is unity whereas the to and to gains are greater than unity, indicating “amplification” ( ). Timing relationships show amplification between to and compression for to and to , ( ). Application of these gains to -3-deficiency indicates that all timing changes are downstream of photoreceptor changes, but a direct pSTR amplitude loss occurs ( ). Application to diabetes indicates widespread inner retinal dysfunction which cannot be attributed to outer retinal changes ( ). Conclusions. This simple approach aids in the interpretation of inner retinal ERG changes by taking into account gain characteristics found between successive ERG components of normal animals.