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BioMed Research International
Volume 2013 (2013), Article ID 802945, 9 pages
On the Structural Context and Identification of Enzyme Catalytic Residues
Department of Medical Informatics, Tzu Chi University, 701 Zhongyang Road, Section 3, Hualien 97004, Taiwan
Received 29 November 2012; Accepted 28 December 2012
Academic Editor: Tun-Wen Pai
Copyright © 2013 Yu-Tung Chien and Shao-Wei Huang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Enzymes play important roles in most of the biological processes. Although only a small fraction of residues are directly involved in catalytic reactions, these catalytic residues are the most crucial parts in enzymes. The study of the fundamental and unique features of catalytic residues benefits the understanding of enzyme functions and catalytic mechanisms. In this work, we analyze the structural context of catalytic residues based on theoretical and experimental structure flexibility. The results show that catalytic residues have distinct structural features and context. Their neighboring residues, whether sequence or structure neighbors within specific range, are usually structurally more rigid than those of noncatalytic residues. The structural context feature is combined with support vector machine to identify catalytic residues from enzyme structure. The prediction results are better or comparable to those of recent structure-based prediction methods.
Understanding the molecular mechanisms of enzyme catalysis is important in studies of various complicated biological processes. The number of protein structures deposited to the Protein Data Bank  has increased rapidly in the past decade. However, the function and catalytic residues of a large fraction of enzymes are not well studied and understood. Experimental methods which are used to identify enzyme catalytic residues, like site-directed mutagenesis, are time consuming and expensive. Computational methods designed to identify catalytic residues are needed to efficiently handle the huge number of proteins whose catalytic sites are not determined.
Many methods have been developed to predict protein catalytic sites based on information extracted from protein seqence and structure. One of the most direct strategies is based on finding homologous enzymes whose function and catalytic residues are already known [2–6]. Catalytic residues of a novel protein are identified by using sequence or structure similarity search with enzymes whose catalytic residues were well annotated. However, there are still limitations for such methods based on homology search. First, homologous enzymes whose function and catalytic sites are already known are needed. Second, proteins of similar tertiary structures do not always have completely identical function . There are also examples showing that proteins of different tertiary structures have the same function .
To directly identify catalytic sites from single protein structure without needing homology information, it is important to study the fundamental differences between catalytic residues and noncatalytic residues. Sacquin-Mora et al.  used the computation of a force constant, that is, the ease of moving a given residue with respect to the other residues in the protein, to identify catalytic residues and found that the catalytic residues usually have higher force constant. Ben-Shimon and Eisenstein  found that the catalytic residues are often located near the small fractions of the exposed residues closest to the center of the protein. Amitai et al.  converted protein to a network in which the residues are vertices and their interactions are edges and showed that the central hubs in the network are usually functional important residues or residues having direct contact with them. Wie et al.  developed a method, Theoretical Microscopic Titration Curves (THEMATICS), which computes residue electrostatic properties from protein structure, to identify catalytic residues. The THEMATICS method was then combined with geometry features derived from protein structure to predict catalytic residues from enzyme structure using a monotonicity-constrained maximum likelihood approach, called Partial Order Optimum Likelihood (POOL) . A more recent method, EXIA , successfully identifies catalytic residues based on residue side chain orientation of single enzyme structure without needing structure or sequence homology information.
In this study, we first analyzed the structural context of catalytic and noncatalytic residues based on their sequence and structure neighbors. We show that catalytic residues are usually located in structurally more rigid environment than noncatalytic residues. The sequence or structural neighboring residues within specific range of catalytic residues have distinct structural features. We further combined the structural context features and support vector machine to identify catalytic residues from protein structure.
2.1. Calculation of Structural Context
The weighted-contact number model (WCN) [15, 16] is used to calculate structural flexibility of residue environment. WCN is highly correlated to experimental B-factor and order parameter of protein structure solved by nuclear magnetic resonance. The WCN of the th residue is based on the distances between the th residue and all the other residues in the enzyme, as in where is total number of residues in the enzyme, and is the distance between th andth residues. The coordinate of Cα atom is used to represent the position of the residue.
There are two types of structural context: sequence neighbor flexibility (SEQ) and structure neighbor flexibility (STR). The SEQ of the th residue is defined as the average structural flexibility of the th residue and its flanking residues on sequence as in where residues to are the nearest neighbors of the th residue on sequence. WCN is inverted for an easy comparison with B-factor. If is out of the range of the sequence, it is simply ignored in the calculation. The STR of the th residue is defined as the average structural flexibility of the th residue and residues whose distance to the th residue are smaller than a cut-off value as in where is a subset of residues whose distance to the th residue is smaller than the cut-off distance and is the number of residues in the subset. The concept of SEQ and STR is extended from our previous work , which only considered the nearest two sequence neighbors.
2.2. Normalization of Structural Context and B-Factor Profiles
The SEQ, STR, and B-factor are normalized to their corresponding -scores: where and are the mean and standard deviations of of a given protein. In this work, is SEQ, STR, or B-factor from X-ray crystallographic structures. For a given protein, the mean and standard deviations are calculated based on the scores of the protein. The scores of the protein are then normalized according to its mean and standard deviations. The normalized SEQ, STR, and B-factor are referred to as , , and , respectively. For convenience, the normalized SEQ, STR, and normalized B-factor profiles are simply called SEQ profile, STR profile and B-factor profile.
2.3. The Support Vector Machine
SVM finds the separating hyperplane with the largest distance between two classes. However, the data being classified may not always be linearly separable in the space. It was proposed that the original space be mapped into a higher-dimensional space, making the separation easier in that space. The support vector machine method (SVM) has been widely applied to many bioinformatics studies: protein-fold assignment [18, 19], subcellular localization prediction [20, 21], secondary-structure prediction [22–24], and other biological pattern-classification problems [25–28]. SVMs perform well in these classification problems when compared to other machine-learning methods because of thier convenient classifier’s capacity control and avoidance of overfitting. In this work, the software package LIBSVM  version 3.11 was used.
Here we used SVM to predict catalytic residues using the structural context features, SEQ and STR, as input features. The feature vector for a residue is one of these features or their combinations: , , , or binary coding of amino acid type. The common problem encountered in enzyme catalytic site prediction using SVM is the extremely unbalanced ratio of catalytic residues and noncatalytic residues. A well-used strategy is to randomly select subsets which have a balanced ratio between catalytic and noncatalytic residues when training . Here, a 5-fold cross-validation procedure was used for performance measurement. For each fold, the training data was a randomly selected balanced subset of residues by subsampling noncatalytic residues. The cost and gamma are parameters used in model training and kernel function of LIBSVM and need to be to tuned for optimal prediction results. These parameters are tuned independently using 5-fold cross-validation for each training dataset. In addition to cost and gamma parameters, other settings used in the SVM include the type of SVM: C-SVC; the type of kernel function: radial basis function. Other parameters not mentioned here are set as their default value in the LIBSVM software.
2.4. Sequence Conservation Score
For comparison with the POOL method, sequence conservation which includes evolutionary information is used as training feature in some predictions reported here. Sequence conservation is from position-specific substitution matrix (PSSM) generated by PSI-Blast  for each protein. PSI-Blast is set to search against the nonredundant (nr) database for three iterations with default -value threshold of 5 × 10−3. The sequence conservation score is directly taken from the “information per position” column in the PSSM profile.
The dataset used in this work is collected from Catalytic Site Atlas (CSA)  version 2.2.10 using BlastClust . The dataset contains 760 proteins with pairwise sequence identity ≤30%, including a total of 592,382 residues in which 2,355 residues are catalytic sites. All heteroatoms, ligands, and nonprotein molecules are removed. The dataset is referred to as E760 dataset.
2.6. Evaluation of Prediction Performance
Sensitivity, specificity, and Matthew’s correlation coefficient (MCC) were used for performance measure as follows: where , , , and are the number of true positive, false positive, true negative, and false negative, respectively. A catalytic residue is either TP when correctly predicted to be catalytic residue or FP when incorrectly predicted to be noncatalytic residue. A noncatalytic residue is either TN when correctly predicted to be noncatalytic residue or FN when incorrectly predicted to be catalytic residue. We used MCC to evaluate the performances because MCC takes into account true and false positives and negatives and is a balanced measure especially when the numbers of positives (catalytic residues) and negatives (noncatalytic residues) are extremely unbalanced. Note that the MCC, sensitivity, and specificity reported here are based on balanced data, that is, the numbers of catalytic and noncatalytic residues are equal. They were only used to compare the results between different features in this paper but not used to compare with other methods. The Receiver Operating Characteristic (ROC) curve was calculated based on unbalanced data and was used to compare prediction results with other methods. The ROC curve was plotted by averaging per-protein ROC curve as used in .
3. Results and Discussions
First, we discuss the distributions of , , and of catalytic residues and noncatalytic residues for the E760 dataset. Then we show the prediction results based on profile, profile, and amino acid type. Finally, we compared the prediction results based on with those of the methods using other structure-based features.
3.1. Distributions of SEQ for Catalytic and Noncatalytic Residues
In this section, we compare the distributions of SEQ (sequence neighbor flexibility) for catalytic residues and noncatalytic residues. Figure 1(a) displays the distributions of when (: the number of flanking neighboring residues on sequence to calculate the average structural flexibility) for catalytic residues and noncatalytic residues for the E760 dataset. For comparison, the distributions of are also shown in Figure 1(b). The distributions of for catalytic and noncatalytic residues show that catalytic residues are much less flexible and located in a more rigid context than noncatalytic residues. The phenomenon is much more significant using than using as shown in Figure 1. There are 90% of catalytic residues having and 40% of noncatalytic residues having . Only 81% of catalytic residues have and 54% of noncatalytic residues have . SEQ and crystallographic B-factor are both based on the number and distances of neighbors around a given residue and are related to structural flexibility. The SEQ profile is a better and more reliable characteristic to identify catalytic residues than the B-factor profile. B-factor is easily affected by experimental conditions, crystal packing, existence of ligands, or temperature. Two structures of the same enzyme under different experimental conditions may have very different B-factor profiles but have almost identical crystal structures.
Figure 2(a) shows the of (solid lines) and (dashed lines) profiles of two enzymes, diaminopimelate epimerase (PDB id: 1BWZ) and levansucrase (PDB id: 1OYG). The catalytic residues and noncatalytic residues are labeled as triangle and circle on profiles and on profiles, respectively. It is obvious that the catalytic residues are located in the most structurally stable regions for the profiles in both examples. The four catalytic residues (Cys73, His159, Glu208, and Cys217) of diaminopimelate epimerase are located near the centroid of the enzyme, forming a rigid catalytic spot. Cys73 and Cys217 are close to each other and connected by a disulfide bond. However, they have unusually high (3.06 and 0.06, resp.) but reasonable low values (−1.08 and −1.22). His159 is partially buried by surrounding neighbors and has a quite low solvent accessible surface (SAS) of 3 Å2, calculated by the DSSP program . It has a relatively low (−0.29) and an extremely low (−1.16) comparing to other residues in the enzyme. Glu208 is relatively more exposed to solvent (SAS = 19 Å2) than His159. It has a high (0.23) but a very low (−0.91). In the protein, the four catalytic residues are structurally rigid, having very low and SAS values. However, their are high, especially for Cys73 that forms a disulfide bond with another catalytic residue, Cys217.
The catalytic site of the second example, levansucrase, is constituted of three catalytic residues, Asp86, Asp247, and Glu342, which are inside a cleft near the geometrical center of the protein. They are moderately accessible to solvent (with SAS: 20 Å2, 8 Å2, and 24 Å2, resp.) but are surrounded and thus strongly stabilized by a large number of residues because of their location. The for these three catalytic residues, Asp86, Asp247, and Glu342, are −1.18, −1.46, and −1.36, respectively. Their values are surprisingly not low (0.56, 0.08, and 1.21, resp.). SEQ is a better estimation of structural flexibility than B-factor whether the location of residue is exposed to solvent or buried inside the protein.
3.2. Distributions of SEQ and STR Based on Different Parameter Settings
In the previous section, we discussed the SEQ when parameter , that is, average WCN of target residue and its nearest two neighboring residues on sequence. Here, we extend the analysis to SEQ calculated based on different window sizes. Figure 3 shows the distributions of SEQ for catalytic and noncatalytic residues with incremental from 1 to 20. When is set to 1, the SEQ distributions of catalytic and noncatalytic residues are obviously different. As window size increases, the differences between distributions decrease gradually. The difference between SEQ of catalytic and noncatalytic residues is less obvious when is larger than 10. Table 2 lists the prediction results when using SEQ with different . The MCC obviously drops when is equal or larger than 10. The results indicate that the sequence neighbors of catalytic residues are also structurally more rigid than noncatalytic residues in the range of .
For STR, the cut-off distance for structurally neighboring residues is set from 3 Å to 25 Å as shown in Figure 4. Catalytic residues have structurally rigid neighbors for neighboring residues within 15 Å cut-off distance. When the cut-off distance is larger than 19 Å, catalytic and noncatalytic residues have similar STR distributions. The results suggest that catalytic residues are usually located in structurally stable environments and the surrounding neighboring residues within 15 Å are also relatively structurally rigid.
3.3. Prediction of Catalytic Residues Based on SEQ and STR
In this section, we discuss the prediction results using SVM with several different features, including amino acid type, SEQ (), STR (cutoff = 3 Å), B-factor profile, and their combinations. The prediction sensitivity, specificity, and MCC using different feature sets are listed in Table 1, including amino acid type (AA), SEQ and STR profiles, B-factor profile (B), combination of amino acid type, and SEQ ().
The prediction results show that SEQ and STR are much better features than B-factor ( for SEQ and STR, 0.25 for B-factor) for identification of catalytic residues. The prediction performances of STR and SEQ are quite similar (sensitivity = 0.76 and 0.79, specificity = 0.70 and 0.69 for SEQ and STR, resp.). We selected SEQ for further-detailed analysis and comparison. Due to the fact that about 95% catalytic residues are polar or charged amino acids, prediction purely based on amino acid type have a MCC of 0.40, which is much higher than that of B-factor. However, the results also show that there are many false positives (specificity = 0.70), which means that catalytic residues have other unique features. SEQ provides information of structural flexibility of residues and their neighbors, which is complementary to amino acid type information. The prediction results that include SEQ and amino acid type show that catalytic residues can be more accurately identified using both features. The MCC is 0.51 and the sensitivity and specificity are 0.74 and 0.76, respectively. The prediction results based on combining SEQ () and STR (cutoff = 3 Å) are also listed in Table 1. The prediction performance is similar to those based on SEQ or STR alone. The reason may be that the combination of SEQ and STR does not provide more information than SEQ or STR alone, thus not further improving the prediction results.
3.4. Comparison with Structure-Based Prediction Method
To test the performance of SEQ, we compared our prediction results with those of Partial Order Optimum Likelihood (POOL) , which combines residue electrostatic properties and structure geometry information to predict catalytic residues. POOL is one of the most successful structure-based prediction methods and it is able to work without needing sequence homology information. First, we directly compared the prediction results of SEQ and those of POOL on a dataset of 160 enzymes . Figure 5 shows the ROC curves of SEQ and POOL based on different features including POOL(T): residue electrostatic properties, POOL(G): structure geometry feature, and POOL(C): sequence conservation. SEQ apparently outperforms POOL(C) and POOL(G) and performs better than POOL(T) and POOL() (POOL(G) combined with POOL(C)) when false-positive rate is smaller than 0.1. Under higher false-positive rates, POOL(T) and POOL() have better performance than that of SEQ. It is somewhat interesting that SEQ, which only uses structural rigidity, has comparable results as those of POOL, which uses residue biochemical features, evolutionary sequence conservation, and cleft shape. The results reported here are based on fivefold cross-validation on the dataset of 160 enzymes. The ratio between catalytic and noncatalytic residues is not changed (unbalanced) for each test fold.
We also compared the results of SEQ and those of POOL() (residue electrostatic combined with structure geometry) on a dataset of 79 enzymes . Figure 6 shows the ROC curves of SEQ (dotted line) and those of POOL. In the results of Figure 5, SEQ performs much better than POOL(G) and is comparable to POOL(T). When POOL(T) and POOL(G) are combined together (POOL()), it performs better than SEQ (Figure 6). POOL has the best performance when sequence conservation (POOL(C)) is further added (POOL()). To compare with their results, we combined SEQ and sequence conservation by PSI-BLAST. The results show that SEQ performs even better than POOL() when sequence conservation is added (thick solid line in Figure 6). It suggests that although SEQ can find out rigid regions in enzyme structures, amino acid information is still important for the identification of catalytic residues due to the fact that a large fraction of catalytic residues are polar amino acids. Using SEQ without any amino acid information to predict catalytic residue may result in some false positives, for example, rigid but nonpolar residues.
To further compare the results of SEQ and POOL, we have submitted two enzyme structures, diaminopimelate epimerase (PDB code: 1BWZ) and levansucrase (PDB code: 1OYG), to the POOL webserver. For diaminopimelate epimerase, the four catalytic residues, Cys73, His159, Glu208, and Cys217, are ranked 18, 3, 6, and 59, respectively, by POOL (residues higher ranked in POOL are more probable to be catalytic residue). In Figure 2(a), it is clear that SEQ correctly identifies all catalytic residues, which are located on the globally most rigid (small SEQ values) regions. For levansucrase, the three catalytic residues, Asp86, Asp247, and Glu342, are ranked 6, 5, and 2, respectively, by POOL. In Figure 2(b), the three catalytic residues locate in the most structurally rigid regions according to SEQ. It is also interesting that residues Glu340, Glu262, and Tyr411 are ranked 1, 3, 4, respectively, by POOL. These residues are located in relatively rigid regions in the SEQ profile. Table 3 lists the prediction rank of POOL and our prediction using SEQ () for each catalytic residue in several example proteins. The rank of our prediction is based on the probability of a residue predicted to be catalytic residue based on the function provided by the LIBSVM software. The results show that our prediction results are in general better than or comparable to those of POOL in these examples.
3.5. Discussions on Related Prediction Methods
Here we discuss related catalytic residue prediction methods, including their features, datasets, and prediction performance. Petrova and Wu  used 24 features, including sequence-based features: amino acid type, sequence conservation, and structure-based and chemical features: shape of local structure, solvent accessible surface, structural flexibility, and hydrogen bonding. A dataset of 79 enzymes containing totally 23,664 residues and 254 catalytic residues was used for performance evaluation. Among these features, the seven best features were selected. The MCC of using different combinations of these features ranges from 0.52 to 0.74 and the sensitivities range from 0.88 to 0.89. To avoid the problems in SVM training due to the extremely unbalanced number of catalytic and noncatalytic residues, they used a similar strategy we used here for SVM training and predicting. The strategy is to build a subset that includes all catalytic residues and equal number of noncatalytic residues selected randomly. It is interesting to note that, without using the sequence conservation feature, the prediction MCC is only 0.52.
A more recent study by Cilia and Passerini  models spherical regions around target residues and extracts the properties of their content such as physicochemical properties, atomic density, flexibility, and presence of water molecules. They performed the prediction using SVM with these structural features and other sequence-based features: amino acid type and sequence conservation.
Amitai et al.  applied graph theory to catalytic residue prediction by converting protein structure to network in which the graph nodes are residues and graph edges are residues interactions. They found that catalytic sites have higher network closeness than noncatalytic residues. The features used in their prediction included the closeness feature, solvent accessible surface, and sequence conservation using a dataset of 178 enzymes.
Wie et al.  used a computational method called Theoretical Microscopic Titration Curves (THEMATICS), which computes theoretical electrostatic properties of residues based on structure information. They simply set a threshold to identify catalytic residues, that is, each residue was assigned a score calculated by THEMACTICS and residues having score greater than the threshold are predicted as catalytic residues. The dataset used contains 169 enzymes, including 594 annotated catalytic residues. The sensitivities using different thresholds range from 0.41 to 0.63. THEMATICS is then combined with other structure feature and is called POOL, with which we chose to compare our results. The reason we compared with POOL is that POOL is the most accurate structure-based prediction method. There are other methods having better prediction results combining complicated sequence features and structure features . However, they usually do not provide prediction results only using structure features.
In this work, we calculated theoretical structural flexibility for catalytic residues and their sequence or structure neighboring residues. We found that catalytic residues are in general located in structurally less flexible context. We show that the theoretical structure flexibility (SEQ and STR) we used is better than B-factor for identification of catalytic residues. For a dataset of 760 enzymes of low pairwise sequence identity, the difference of SEQ distributions between catalytic and noncatalytic residues are more obvious than that of B-factor. The prediction results of SEQ are much better then those of B-factor. The MCC, sensitivity, and specificity of prediction are 0.74, 0.76, and 0.51, respectively, using SEQ combined with amino acid type information. The prediction results using SEQ are comparable to or better than those of other structure-based features. Most current prediction methods need homology information, for example, sequence conservation from PSI-Blast, and require the existence of sequence or structure similar proteins. SEQ and STR are calculated from single-protein structure and do not require any homology information. They may be further applied to the detection of enzyme function-related sites, like protein ligand binding site, metal binding site, or protein-protein interaction hotspot residues.
This research was supported by Grant (101-2218-E-320-001-) of National Science Council, Taiwan.
- H. M. Berman, J. Westbrook, Z. Feng et al., “The protein data bank,” Nucleic Acids Research, vol. 28, no. 1, pp. 235–242, 2000.
- J. A. Capra and M. Singh, “Predicting functionally important residues from sequence conservation,” Bioinformatics, vol. 23, no. 15, pp. 1875–1882, 2007.
- D. La, B. Sutch, and D. R. Livesay, “Predicting protein functional sites with phylogenetic motifs,” Proteins, vol. 58, no. 2, pp. 309–320, 2005.
- M. Ota, K. Kinoshita, and K. Nishikawa, “Prediction of catalytic residues in enzymes based on known tertiary structure, stability profile, and sequence conservation,” Journal of Molecular Biology, vol. 327, no. 5, pp. 1053–1064, 2003.
- B. Sterner, R. Singh, and B. Berger, “Predicting and annotating catalytic residues: an information theoretic approach,” Journal of Computational Biology, vol. 14, no. 8, pp. 1058–1073, 2007.
- J. W. Torrance, G. J. Bartlett, C. T. Porter, and J. M. Thornton, “Using a library of structural templates to recognise catalytic sites and explore their evolution in homologous families,” Journal of Molecular Biology, vol. 347, no. 3, pp. 565–581, 2005.
- N. Nagano, C. A. Orengo, and J. M. Thornton, “One fold with many functions: the evolutionary relationships between TIM barrel families based on their sequences, structures and functions,” Journal of Molecular Biology, vol. 321, no. 5, pp. 741–765, 2002.
- A. C. Wallace, R. A. Laskowski, and J. M. Thornton, “Derivation of 3D coordinate templates for searching structural databases: application to Ser-His-Asp catalytic triads in the serine proteinases and lipases,” Protein Science, vol. 5, no. 6, pp. 1001–1013, 1996.
- S. Sacquin-Mora, E. Laforet, and R. Lavery, “Locating the active sites of enzymes using mechanical properties,” Proteins, vol. 67, no. 2, pp. 350–359, 2007.
- A. Ben-Shimon and M. Eisenstein, “Looking at enzymes from the inside out: the proximity of catalytic residues to the molecular centroid can be used for detection of active sites and enzyme-ligand interfaces,” Journal of Molecular Biology, vol. 351, no. 2, pp. 309–326, 2005.
- G. Amitai, A. Shemesh, E. Sitbon et al., “Network analysis of protein structures identifies functional residues,” Journal of Molecular Biology, vol. 344, no. 4, pp. 1135–1146, 2004.
- Y. Wei, J. Ko, L. F. Murga, and M. J. Ondrechen, “Selective prediction of interaction sites in protein structures with THEMATICS,” BMC Bioinformatics, vol. 8, article 119, 2007.
- W. Tong, Y. Wei, L. F. Murga, M. J. Ondrechen, and R. J. Williams, “Partial Order Optimum Likelihood (POOL): maximum likelihood prediction of protein active site residues using 3D structure and sequence properties,” PLoS Computational Biology, vol. 5, no. 1, Article ID e1000266, 2009.
- Y. T. Chien and S. W. Huang, “Accurate prediction of protein catalytic residues by side chain orientation and residue contact density,” PLoS ONE, vol. 7, Article ID e47951, 2012.
- S. W. Huang, C. H. Shih, C. P. Lin, and J. K. Hwang, “Prediction of NMR order parameters in proteins using weighted protein contact-number model,” Theoretical Chemistry Accounts, vol. 121, no. 3-4, pp. 197–200, 2008.
- C. P. Lin, S. W. Huang, Y. L. Lai et al., “Deriving protein dynamical properties from weighted protein contact number,” Proteins, vol. 72, no. 3, pp. 929–935, 2008.
- Y. T. Chien and S. W. Huang, “Prediction of protein catalytic residues by local structural rigidity,” in Proceedings of the 6th International Conference on Complex, Intelligent and Software Intensive Systems (CISIS '12), pp. 592–596, Palermo, Italy, 2012.
- C. H. Q. Ding and I. Dubchak, “Multi-class protein fold recognition using support vector machines and neural networks,” Bioinformatics, vol. 17, no. 4, pp. 349–358, 2001.
- C. S. Yu, J. Y. Wang, J. M. Yang, P. C. Lyu, C. J. Lin, and J. K. Hwang, “Fine-grained protein fold assignment by support vector machines using generalized npeptide coding schemes and jury voting from multiple-parameter sets,” Proteins, vol. 50, no. 4, pp. 531–536, 2003.
- C. S. Yu, C. J. Lin, and J. K. Hwang, “Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions,” Protein Science, vol. 13, no. 5, pp. 1402–1406, 2004.
- S. Hua and Z. Sun, “Support vector machine approach for protein subcellular localization prediction,” Bioinformatics, vol. 17, no. 8, pp. 721–728, 2001.
- S. Hua and Z. Sun, “A novel method of protein secondary structure prediction with high segment overlap measure: support vector machine approach,” Journal of Molecular Biology, vol. 308, no. 2, pp. 397–407, 2001.
- H. Kim and H. Park, “Protein secondary structure prediction based on an improved support vector machines approach,” Protein Engineering, vol. 16, no. 8, pp. 553–560, 2003.
- B. Rost and C. Sander, “Prediction of protein secondary structure at better than 70% accuracy,” Journal of Molecular Biology, vol. 232, no. 2, pp. 584–599, 1993.
- Y. C. Chen and J. K. Hwang, “Prediction of disulfide connectivity from protein sequences,” Proteins, vol. 61, no. 3, pp. 507–512, 2005.
- Y. C. Chen, Y. S. Lin, C. J. Lin, and J. K. Hwang, “Prediction of the bonding states of cysteines using the support vector machines based on multiple feature vectors and cysteine state sequences,” Proteins, vol. 55, no. 4, pp. 1036–1042, 2004.
- S. W. Huang and J. K. Hwang, “Computation of conformational entropy from protein sequences using the machine-learning method—application to the study of the relationship between structural conservation and local structural stability,” Proteins, vol. 59, no. 4, pp. 802–809, 2005.
- H. Kim and H. Park, “Prediction of protein relative solvent accessibility with support vector machines and long-range interaction 3D local descriptor,” Proteins, vol. 54, no. 3, pp. 557–562, 2004.
- C. C. Chang and C. J. Lin, LIBSVM: a library for support vector machines, 2001, http://www.csie.ntu.edu.tw/~cjlin/libsvm.
- Y. R. Tang, Z. Y. Sheng, Y. Z. Chen, and Z. Zhang, “An improved prediction of catalytic residues in enzyme structures,” Protein Engineering, Design and Selection, vol. 21, no. 5, pp. 295–302, 2008.
- S. F. Altschul, T. L. Madden, A. A. Schäffer et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucleic Acids Research, vol. 25, no. 17, pp. 3389–3402, 1997.
- C. T. Porter, G. J. Bartlett, and J. M. Thornton, “The Catalytic Site Atlas: a resource of catalytic sites and residues identified in enzymes using structural data,” Nucleic Acids Research, vol. 32, pp. D129–D133, 2004.
- W. Kabsch and C. Sander, “Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features,” Biopolymers, vol. 22, no. 12, pp. 2577–2637, 1983.
- N. V. Petrova and C. H. Wu, “Prediction of catalytic residues using Support Vector Machine with selected protein sequence and structural properties,” BMC Bioinformatics, vol. 7, article 312, 2006.
- E. Cilia and A. Passerini, “Automatic prediction of catalytic residues by modeling residue structural neighborhood,” BMC Bioinformatics, vol. 11, article 115, 2010.