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Figure 1: Construction of recombinant hR expression vector and transfection in cell lines. (A) A full length of hR coding sequence was inserted into PcDNA-3.1 (Pc-3.1). (B) The expression of hR mRNA was identified in C2C12 cells transfected with PcDNA-hR (Pc-hR). (C) Immunocytochemistry analysis showed hR immunoreactive protein in C2C12 cells transfected with Pc-hR. (D) Western blot analysis showed expression of hR in COS7 cells transfected with Pc-hR. C2C12-Pc-3.1: C2C12 cells transfected with Pc-3.1 plasmids; C2C12-Pc-hR: C2C12 cells transfected with Pc-hR plasmids.