In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells
Figure 2
Characterization of cord blood-derived erythroid progenitor cells. ((a)–(c)) Photographs of cytospin preparations of the erythroid progenitor cells stained by Wright-Giemsa. CD34+ cells (a), and day 10 (b) and day 15 (c) erythroid progenitors were cytospined on a polylysine-coated slide and stained with Wright-Giemsa reagents. Original magnification: ×400. ((d) and (e)) Methyl-cellulose assays of 21 days erythroid cells. (f) Erythroid progenitor cells (pellet) cultured for 21 days in vitro. Left is PBS as control.