In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells
Figure 5
Enucleation of cord blood HSCs-derived erythroid cells in vitro. ((a) and (b)) Erythroid progenitors cocultured with hFLSCs for 4 days (a) and 8 days (b). Bars = 100 μm. ((c) and (d)) Erythroid cells derived from human cord blood HSCs were cocultured with hFLSCs for 8 days. On day 4 (c) and 8 (d), cells were cytospun and stained with Wright-Giemsa dye. Bars = 100 μm. (e) Maturation of cord blood-derived erythroid cells mimic erythroid development. Expression of GPA, a mature erythrocyte marker, increases with time and CD71, an immature red blood cell marker, shows a decrease in expression over time.
