In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells
Table 1
Flow cytometry analysis of cell surface markers during erythroid progenitor cells differentiation from cord blood derived HSCs (mean ± SD of triplicate assays).
Marker
7 day (%)
14 day (%)
21 day (%)
CD3*
1.78 ± 0.23
0.14 ± 0.08
0.42 ± 0.26
CD8*
1.14 ± 0.19
1.76 ± 0.40
2.31 ± 0.98
CD14*
6.20 ± 1.48
1.4 ± 0.29
2.11 ± 1.20
CD33*
81.23 ± 2.17
66.02 ± 1.93
59.41 ± 4.08
CD38*
30.65 ± 4.42
3.28 ± 0.85
0.84 ± 0.31
CD45
97.15 ± 1.43
96.28 ± 1.78
96.31 ± 0.80
GPA*
1.15 ± 0.51
7.49 ± 1.32
14.92 ± 2.12
CD4*
1.39 ± 0.28
1.16 ± 0.59
3.60 ± 1.27
CD13*
26.40 ± 1.86
4.72 ± 1.51
8.20 ± 1.26
CD19
1.21 ± 0.22
0.08 ± 0.04
0.04 ± 0.03
CD34
0.53 ± 0.11
0.50 ± 0.41
0.64 ± 0.25
CD41*
4.24 ± 1.21
3.18 ± 0.76
2.55 ± 1.57
CD71*
73.46 ± 2.89
96.32 ± 1.44
94.44 ± 0.67
CD117*
63.56 ± 7.05
83.57 ± 3.13
88.72 ± 2.63
The cell surface markers expression of cultured erythroid progenitor cells significantly changed. The expression of CD4, CD8, CD71, CD117, and GPA significantly increased, while the CD3, CD13, CD14, CD33, CD38, and CD41 significantly decreased (* value < 0.01, using Student’s -test).
