810734.fig.004a
(a) Untreated cells
810734.fig.004b
(b) ET 100 μg/mL
810734.fig.004c
(c) Camptothecin 5 μM
Figure 4: Loss of mitochondrial membrane potential ( ) in P. hysterophorus leaf ET extract treated HL-60 cells. (a) Untreated cells (Control), (b) treated cells, and (c) positive control. HL-60 cells (1 × 106/mL/well) were incubated with indicated doses of ethanol (ET) extract and camptothecin for 24 h. Cells were stained with Rhodamine-123 (200 nM) for 1 h and analyzed in FL-1 versus FL-2 channels of flow cytometer as described in materials and methods section. Data are representative of one of three similar experiments.