810734.fig.005a
(a) Untreated cells
810734.fig.005b
(b) ET 100 μg/mL
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(c) Camptothecin 5 μM
Figure 5: Cell cycle analysis of P. hysterophorus leaf ET extract treated HL-60 cells. (a) Untreated cells (control), (b) treated cells, and (c) Positive control. HL-60 cells (2 × 106 cells/mL/well) were exposed to the indicated concentrations of ethanol (ET) extract and camptothecin for 24 h and stained with propidium iodide to determine DNA fluorescence and cell cycle phase distribution as described in materials and methods section. Fraction of cells for sub-G1 population analyzed from FL-2 versus cell counts is shown. Data are representative of one of three similar experiments.