Analytical Variables Affecting Analysis of F₂-Isoprostanes and F₄-Neuroprostanes in Human Cerebrospinal Fluid by Gas Chromatography/Mass Spectrometry

Table 2

Results of quantification of F₄-NPs from 5 consecutive injections of the same pooled sample.

	F4-NPs levels (pg/mL)
	No additional holding	30-min holding
CSF-1	119.9	113.8
CSF-2	130.2	114.0
CSF-3	144.4	117.7
CSF-4	145.5	119.3
CSF-5	147.5	121.7
Mean ± SD	137.5±12.0	117.3±3.4^∗
(CV%)	(8.7%)	(2.9%)

The sample number was designated according to the sequence of injection. The quantification data for samples analyzed without additional holding corresponds to chromatograms shown by the left panel of Figure 6, whereas that with 30-min holding corresponds to chromatograms shown by the right panel of Figure 6. CV is coefficient of variation that is the ratio of standard deviation (SD) to mean. *𝑝<0.05 (𝑝=0.017 under assumption of equal variance) between two sets of data obtained by two different methods.