Table 2: Results of quantification of F4-NPs from 5 consecutive injections of the same pooled sample.

F4-NPs levels (pg/mL)
No additional holding30-min holding

CSF-1119.9113.8
CSF-2130.2114.0
CSF-3144.4117.7
CSF-4145.5119.3
CSF-5147.5121.7

Mean ± SD
(CV%)(8.7%)(2.9%)

The sample number was designated according to the sequence of injection. The quantification data for samples analyzed without additional holding corresponds to chromatograms shown by the left panel of Figure 6, whereas that with 30-min holding corresponds to chromatograms shown by the right panel of Figure 6. CV is coefficient of variation that is the ratio of standard deviation (SD) to mean. * ( under assumption of equal variance) between two sets of data obtained by two different methods.