Phytoestrogen α-Zearalanol Attenuates Homocysteine-Induced Apoptosis in Human Umbilical Vein Endothelial Cells
Figure 2
Pretreatment with α-ZAL improved the deceased cell viability induced by homocysteine with methyl thiazolyl-tetrazolium (MTT) assay in HUVECs. (a) Treatment with different concentrations of homocysteine on HUVECs for 24 h decreased cell viability in a dose-dependent manner, which became apparent at 500 μmol/L. (b) Treatment with 500 μmol/L homocysteine on HUVECs decreased cell viability in a time-dependent manner, which became apparent at 24 h. (c) Pretreatment with α-ZAL or 17β-E2 (10−8~10−6 mol/L) could significantly improve the decreased cell viability induced by homocysteine (500 μmol/L, 24 h), which was similar to 17β-E2. Data were presented as mean ± SD. versus Vehicle, versus Hcy alone. α-ZAL: α-zearalanol; 17β-E2: 17β-estradiol; Hcy: homocysteine.
