Phytoestrogen α-Zearalanol Attenuates Homocysteine-Induced Apoptosis in Human Umbilical Vein Endothelial Cells

Figure 6

Pretreatment with α-ZAL reduced the expression of proapoptotic protein Bax in homocysteine-challenged HUVECs. The expression of proapoptotic protein Bax was significantly increased after treatment with 500 μmol/L homocysteine for 24 h. Pretreatment with α-ZAL could reduce the expression of Bax. (a) Immunohistochemistry staining (only showing the picture of the highest concentration 10⁻⁶ mol/L). (b) Western blot. Data were presented as mean ± SD. ^∗𝑃<0.05 versus Vehicle, ^#𝑃<0.05 versus Hcy alone. α-ZAL: α-zearalanol; 17β-E₂: 17β-estradiol; Hcy: homocysteine.

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