Research Article
Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
Table 3
Electrophoretic banding analysis of seeds storage proteins yielded from irradiated Vicia faba plant by various doses of FN and UV-B.
| | No. | MW/KDa |
Protein marker | Fluencies of FN radiation (n/cm²) |
Un-irritated
sample | Various doses of UV-B radiation (μw/cm²) |
Polymorphism types | | | 2.5 × 105 | 2.3 × 106 | 3.0 × 107 | 1.5 × 108 | | 1/2 h | 1 h | 2 h | 3 h | | Lane M | Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | | KDa | % | KDa | % | KDa | % | KDa | % | KDa | % | KDa | % | KDa | % | KDa | % | KDa | % | KDa | % |
| | 1 | 315 | + | + | + | 32.77 | + | 106.58 | + | 106.84 | + | 66.82 | + | 87.95 | + | 90.73 | + | 101.3 | + | 96.49 | + | 52.91 | M | | 2 | 181 | − | − | | | − | − | − | − | − | − | + | 32.69 | − | − | − | − | − | − | − | − | P | | 3 | 175 | − | − | − | − | + | 17.83 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | U | | 4 | 158 | − | − | − | − | − | − | + | 16.35 | − | − | − | − | + | 11.28 | + | 17.83 | + | 12.57 | − | − | P | | 5 | 145 | − | − | − | − | − | − | − | − | + | 14.3 | − | − | − | − | + | 10.37 | − | − | − | − | P | | 6 | 128 | − | − | − | − | + | 17.83 | − | − | − | − | + | 15.39 | − | − | − | − | − | − | − | − | P | | 7 | 120 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | 9.43 | − | − | − | − | U | | 8 | 116 | 116 | 8.86 | − | − | + | 10.54 | + | 15.16 | − | − | − | − | + | 11.28 | + | 12.48 | − | − | − | − | P | | 9 | 97 | 97 | 29.84 | − | − | − | − | − | − | + | 9.23 | − | − | − | − | − | − | + | 9.43 | − | − | P | | 10 | 86 | − | − | | | + | 106.00 | + | 125.86 | + | 5.34 | + | 99.46 | − | − | − | − | − | − | − | − | P | | 11 | 75 | − | − | + | 81.51 | + | 103.47 | − | − | − | − | + | 99.23 | + | 114.7 | + | 118.96 | − | − | + | 9.22 | P | | 12 | 66 | 66 | 33.00 | + | 77.69 | + | 118.97 | + | 106.00 | − | − | + | 105.23 | + | 111.6 | + | 118.96 | − | − | + | − | P | | 13 | 60 | − | − | − | − | − | − | − | − | − | − | + | 128.02 | − | − | − | − | − | − | − | 7.22 | p | | 14 | 54 | − | − | + | 77.69 | + | 95.21 | − | − | − | − | + | 126.51 | − | − | + | − | − | − | − | − | P | | 15 | 50 | − | − | + | 91.41 | + | 94.02 | + | 86.79 | − | − | + | 117.37 | − | − | + | 126.51 | − | − | − | − | P | | 16 | 45 | − | − | + | 52.69 | + | 101.95 | + | 125.54 | + | 100.21 | + | 138.29 | + | 109.63 | + | 77.41 | + | 58.55 | + | 14.84 | M | | 17 | 40 | − | − | + | 14.84 | − | − | + | 38.65 | + | 73.35 | + | 56.23 | + | 55.71 | + | 77.19 | − | − | + | 28.59 | P | | 18 | 38 | 38 | 32.88 | + | 21.36 | + | 14.57 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | P | | 19 | 29 | − | − | − | − | + | 11.62 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | U | | 20 | 25 | − | − | + | 225.17 | + | 232.77 | + | 266.37 | + | 256.43 | + | 267.16 | + | 261.93 | + | 237.79 | + | 231.78 | + | − | p | | 21 | 23 | − | − | − | − | + | 19.42 | + | 41.76 | + | 77.98 | − | − | + | 78.44 | + | 35.64 | + | 36.11 | − | 118.23 | P | | 22 | 18 | 18 | 43.44 | + | 41.76 | + | 56.82 | + | 94.7 | + | 44.88 | − | − | + | 61.21 | − | − | + | 29.95 | − | − | P | | 23 | 16 | − | − | + | 16.23 | + | 26.58 | + | 17.85 | + | 17.34 | + | 9.28 | + | 12.48 | + | 10.46 | + | 29.95 | − | − | P | | 24 | 14 | − | − | − | − | + | 26.58 | − | − | − | − | − | − | − | − | + | 11.07 | − | − | − | − | P | | 25 | 12 | 12 | 103.15 | + | 134.29 | + | 173.34 | + | 188.58 | + | 174.19 | + | 177.85 | + | 179.34 | + | 137.12 | + | 146.44 | + | 128.09 | M |
| | Total bands of each lane | 12 | 18 | 13 | 11 | 14 | 12 | 15 | 9 | 7 | | | % Total bands | 10.81 | 16.22 | 11.71 | 9.91 | 12.61 | 10.81 | 13.51 | 8.11 | 6.31 | |
| | | Polymorphic bands | Unique bands | Monomorphic bands | % of polymorphism | | | Total protein bands of all lanes = 111 | No. of bands | % | No. of bands | % | No. of bands | % | | | | | | | | | | | | | | | | 88 | | | | 22 | 19.82 | 3 | 2.70 | 3 | 2.70 | | |
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