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BioMed Research International
Volume 2013 (2013), Article ID 824682, 8 pages
http://dx.doi.org/10.1155/2013/824682
Research Article

Gastric Tissue Damage Analysis Generated by Ischemia: Bioimpedance, Confocal Endomicroscopy, and Light Microscopy

1Departamento de Procesos y Tecnologia, Universidad Autonoma Metropolitana, Cuajimalpa. Artificios 40, Colonia Hidalgo, 01120 Ciudad de Mexico, DF, Mexico
2Centro Nacional de Investigación en Instrumentacion e Imagenologia Medica, Universidad Autonoma Metropolitana, Iztapalapa. Avenida San Rafael Atlixco 186, Colonia Vicentina, 09340 Ciudad de Mexico, DF, Mexico
3Departamento de Ingenieria Electrica, Universidad Autonoma Metropolitana, Iztapalapa. Avenida San Rafael Atlixco 186, Colonia Vicentina, 09340 Ciudad de Mexico, DF, Mexico
4Departamento de Biologia de la Reproduccion, Universidad Autonoma Metropolitana, Iztapalapa. Avenida San Rafael Atlixco 186, Colonia Vicentina, 09340 Ciudad de Mexico, DF, Mexico

Received 2 May 2013; Accepted 4 June 2013

Academic Editor: Andrea Vecchione

Copyright © 2013 Nohra E. Beltran et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The gastric mucosa ischemic tissular damage plays an important role in critical care patients’ outcome, because it is the first damaged tissue by compensatory mechanism during shock. The aim of the study is to relate bioimpedance changes with tissular damage level generated by ischemia by means of confocal endomicroscopy and light microscopy. Bioimpedance of the gastric mucosa and confocal images were obtained from Wistar male rats during basal and ischemia conditions. They were anesthetized, and stain was applied (fluorescein and/or acriflavine). The impedance spectroscopy catheter was inserted and then confocal endomicroscopy probe. After basal measurements and biopsy, hepatic and gastric arteries clamping induced ischemia. Finally, pyloric antrum tissue was preserved in buffered formaldehyde (10%) for histology processing using light microscopy. Confocal images were equalized, binarized, and boundary defined, and infiltrations were quantified. Impedance and infiltrations increased with ischemia showing significant changes between basal and ischemia conditions ( ). Light microscopy analysis allows detection of general alterations in cellular and tissular integrity, confirming gastric reactance and confocal images quantification increments obtained during ischemia.