Table 1: Hot plate method: effects of methanolic extracts of Nyctanthes arbor-tristis stem barks and morphine on pain induced by hot plate method.

GroupDose (mg/kg)Reaction time (in sec)
0 min15 min30 min60 min90 min

Control4.05 ± 0.013.89 ± 0.164.02 ± 0.324.11 ± 0.023.78 ± 0.15
Standard5 (i.p)4.01 ± 0.12 a*4.92 ± 0.18 a@7.15 ± 0.11 a@8.87 ± 0.12 a@10.41 ± 0.01 a@
MENA250250 (p.o)3.68 ± 0.005 a#b#4.25 ± 0.01 a*b#4.98 ± 0.12 a#b@5.57 ± 0.17 a@b@6.13 ± 0.20 a@b@
MENA500500 (p.o)4.03 ± 0.02 a*b*c#4.68 ± 0.05 a#b*c*6.33 ± 0.10 a@b*c@7.18 ± 0.005 a@b@c@8.02 ± 0.12 a@b@c@

Data are expressed as Mean ± SEM, animals in each group. One-way ANOVA was carried out using Turkey’s multiple comparison tests. Comparisons were made between:
a: Control versus Standard, MENA250, and MENA500.
b: Standard versus MENA250 and MENA500.
c: MENA250 versus MENA500.
Symbols represent statistical significance: * , # , @ .