Table 2: Tail flick assay: time of measurement of latency of tail flick (sec).

GroupDose (mg/kg)Reaction time in (sec)
0 min15 min30 min60 min90 min

Control2.74 ± 0.162.81 ± 0.122.97 ± 0.183.01 ± 0.052.82 ± 0.12
Standard5 (i.p)2.90 ± 0.12 a@4.12 ± 0.18 a@6.59 ± 0.11 a@9.35 ± 0.10 a@9.18 ± 0.18 a@
MENA250250 (p.o)2.80 ± 0.12 a@b@2.99 ± 0.20 ansb@3.67 ± 0.17 a*b@5.47 ± 0.20 a@b@5.11 ± 0.06 a@b@
MENA500500 (p.o)2.85 ± 0.10 a@b*c@3.55 ± 0.12 a*bnscns5.29 ± 0.12 a@b@c@7.75 ± 0.23 a@b@c@6.92 ± 0.16 a@b@c@

Data are expressed as Mean ± SEM, animals in each group. One-way ANOVA was carried out using Turkey’s multiple comparison tests. Comparisons were made between:
a: Control versus Standard, MENA250, and MENA500.
b: Standard versus MENA250 and MENA500.
C: MENA250 versus MENA500.
Symbols represent statistical significance: * , # , @ , ns: nonsignificant.