About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 827621, 16 pages
http://dx.doi.org/10.1155/2013/827621
Review Article

Class A -Lactamases as Versatile Scaffolds to Create Hybrid Enzymes: Applications from Basic Research to Medicine

1Laboratory of Enzymology and Protein Folding, Centre for Protein Engineering, Institute of Chemistry, University of Liege, (Sart-Tilman) 4000 Liege, Belgium
2ProGenosis, Boulevard du Rectorat, 27b-B22, (Sart-Tilman) 4000 Liege, Belgium
3Laboratory of Biological Macromolecules, Centre for Protein Engineering, Institute of Chemistry, University of Liege, (Sart-Tilman) 4000 Liege, Belgium

Received 31 May 2013; Accepted 4 July 2013

Academic Editor: Bidur Prasad Chaulagain

Copyright © 2013 Céline Huynen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Designing hybrid proteins is a major aspect of protein engineering and covers a very wide range of applications from basic research to medical applications. This review focuses on the use of class A β-lactamases as versatile scaffolds to design hybrid enzymes (referred to as β-lactamase hybrid proteins, BHPs) in which an exogenous peptide, protein or fragment thereof is inserted at various permissive positions. We discuss how BHPs can be specifically designed to create bifunctional proteins, to produce and to characterize proteins that are otherwise difficult to express, to determine the epitope of specific antibodies, to generate antibodies against nonimmunogenic epitopes, and to better understand the structure/function relationship of proteins.