BioMed Research International

Review Article

Class A -Lactamases as Versatile Scaffolds to Create Hybrid Enzymes: Applications from Basic Research to Medicine

Table 1

Properties of the different BHPs discussed in this review.


Hybrid proteins ref	Insert		Effect on stability (—kJ·mol⁻¹)	Effects on the β-lactamase activity	Function of the insert
Hybrid proteins ref	Name + size	Insertion site	Effect on stability (—kJ·mol⁻¹)	Effects on the β-lactamase activity	Function of the insert

TEM-1-STa [14, 15]	STa 18 AA	37, 195, 197, 198, 206, 216, 218, 232 and 260	ND	Insertion at positions 37, 198, and 206: ND Insertion at position 195: no loss of activity Insertion at positions 197, 216, 232, and 260: partial loss of activity	Enterotoxicity

BlaP-HA [11]	HA 9 AA	197	ND	No loss of activity	Binding to anti-HA mab: = 0.68 nM

BlaP-ChBD [10]	ChBD 72 AA	197	~3.2	No loss or partial loss of activity depending on the substrate	Binding to chitin: = 5.4 ± 0.5 L g⁻¹
BlaP-ChBD [10]	Thb-ChBD-Thb	197	ND	Partial loss of activity	Binding to chitin

BlaP-CD22 [12]	CD22/C2C3 202 AA	197	ND	Activity detected but not quantified	Binding to mab 8015: = 26.2 ± 1.4 nM

BlaP₁₉₇(Gln [16]	PolyQ tracts 23Q-30Q-55Q and 79Q	197	~7.6–8.8	No loss of activity	PolyQ tracts longer than a threshold trigger the aggregation of BHPs into amyloid fibrils

The name of the BHPs, the name of the insert, its size, and function are indicated. The effects of the insertion on the stability and on the enzymatic activity of the β-lactamase are also reported. ND: not determined.