High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties
Figure 2
(a) SDS-PAGE screening of high expression recombinant strain. M: molecular weight protein marker; N: negative control GS115/pPIC9K; lanes 1–21: expressed supernatants GS115/pPIC9K-N-APP; (b) Western blot analysis of recombinant proteins. M: prestained protein molecular weight marker; lanes 1–21: expressed supernatants GS115/pPIC9K-N-APP; N: negative control GS151/pPIC9K.