Table 1: Schematic summary of the best characterized mAbs against arboviral pathogens.

mAbOriginReactivityTarget proteinEpitopeCloning strategyFormat/isotypeNeutralizing activityReference

4.8AHumanDENV1–4EDIIEBV transformationIgGIC50 (μg/mL): 
DENV1: 1.5
DENV2: >40
DENV3: 2.4
DENV4: >40
[37]
D11CIC50 (μg/mL):  
DENV1: 1.5
DENV2: 1.0
DENV3: 10.2
DENV4: 1.6
1.6DIC50 (μg/mL):  
DENV1: 1.5
DENV2: 0.2
DENV3: 0.5
DENV4: 2.7

2H12MurineDENV1–4EDIII (AB loop: aa 314–317)Hybridoma from BALB/c mouse immunized with DENV2 E/DIIIIgG2bIC50 nM: 
DENV1: 0.56–54
DENV3: 29
DENV4: 145
[38]

C9Murine/chimericDENV2EDIIIPhage display of a chimeric murine hybridoma libraryVH1/Vκ1PRNT50 
850 μg/mL
[39]

4E11MurineDENV1–4EDIII (strand A: 308, 312 and strand G: 387, 389, 391)HybridomaIgG2a/κIC50 (μg/mL):  
DENV10.16
DENV2: 0.13
DENV-3: 8
DENV-4: 15
[40]

4G2MurineDENV1–4EDII (fusion loop)Hybridoma from DENV2 E immunized miceIgGPRNT50 (μg/mL):  
DENV2: 15
[39]

9F12MurineDENV1–4, WNVEDIII (aa 305, 307, 310, 330; A strand and BC loop)Hybridoma from BALB/c mouse immunized with DENV-2 E/DIIIIgG1kDENV1–4PRNT50: 
 M
[41]

2A10G6MurineDENV1–4, YFV, WNV, JEV, TBEVEDII (Fusion loop: aa 98–101)Hybridoma from BALB/c mouse immunized with inactivated DENV2IgG1PRNT50 (μg/mL):  
DENV1: 2
DENV2: 1.5
DENV3: 2.1
DENV4: 1.8
YNF: 3.6
WNV: 46
[36]

mAb11HumanDENV1–4, WNV, SLEV, YFV, JEV MVEVEDII (fusion loop)Phage display of human scFvFusion protein scFv-FcPRNT80 (μg/mL):  
WNV: 1.25
DENV2: 6.25
[42, 43]

E16
(MGAWN1)
MurineWNVEDIII (LR; aa 302–309)Hybridoma from immunized mice with WNV EIgG2b/humanizedPRNT50: 4–18 ng
PRNT90: 53–297 ng
[44, 45]

1A1D-2MurineDENV1–3EDIII (A strand: aa 307, 310 and 312)Hybridoma from immunized mice with different pH-treated virusIgG2aDENV2 PRNT50: 
2.1 nM
[46, 47]

1F4HumanDENV1E (virion) DI-DIIElectrofusion of infected memory B cells from DENV-immune subjects with EBVIgGDENV1 IC50
0.11 μg/mL
[48]
2D22DENV2DIIIDENV2 IC50
0.08 μg/mL
5J7DENV3DI-DIIDENV3 IC50
0.10 μg/mL

E105MurineDENV1EDIII (BC loop: G328, T329 and D330; DE loop: K361E and E362K; FG loop: K385)
Hybridomas derived from C57BL/6 IFN-α βR−/− mice infected with DENV1IgGPRNT50 DENV-1: 0.5–59.2 ng/mL[32]
E106DIII (BC loop: G328, T329 and D330; DE loop: K361E and E362K; FG loop: K385; A strand: S305, K307, E309, K310, and E311)
PRNT50 DENV-1: 0.6–59.2 ng/mL
E111DIII (CC′ loop)PRNT50:  
3.8–25 μg/mL
[49]

CR4374HumanWNVEDIIIPhage display of scFv IgG libraryVH2-05/VL1ePRNT50: 
0.18 μg/mL
PRNT90:  
0.95 μg/mL
[50]
CR4353VH3-30/Vk3-A27PRNT50: 
0.026 μg/mL
PRNT90 
36.4 μg/mL

1A5ChimpanzeeDENV1–4, WNV, JEV, LGTVEDII (aa G106)Phage display of Fab library from DENV1–4 infected chimpanzeesHumanized IgG1PRNT50 (μg/mL):  
DENV1: 0.48
DENV2: 0.95
DENV3: 3.2
DENV4: 4.3
WNV/DENV4: 3.8
JEV: 21
LGTV: 28
[51]

mAb11HumanDENV1–4, WNVEDII (fusion loop, W101, G104, G106)scFv libraryscFv-FcPRNT80 (μg/mL):  
DENV2: 6.25
WNV: 1.25
[42, 43]

3B4C-4MurineSLEVE1aHybridomaIgG SLEV PRNT: <1.7[52, 53]
1B2C-5SLEV1bSLEV PRNT: <1.7
6B5A-2SLEV1cSLEV PRNT: 4.8
4A4C-4SLEV1dSLEV PRNT: 2.9
1B5D-1SLEV, JEV2SLEV PRNT: <1.7
2B5B-3SLEV, JEV, MVEV, WNV, YFV3SLEV PRNT: 2.3
2B6B-2All Flavivirus 4aSLEV PRNT: <1.7
6B6C-1All Flavivirus 4b (in DII)SLEV PRNT: 2.3

A3ChimpanzeeJEVE2DI aa. K179Phage displayHumanizedPRNT50
0.04–0.2 nM
[54]
B2DII aa. I126Phage displayPRNT50
0.02–2 nM
E3DIII aa. G132Phage displayPRNT50
0.14–0.93 nM

FabTJE12B02HumanJEVEN/APhage displayFabFRNT50 
50.2 µg/mL
[55]

5F10HumanCHIKVE2Domain BEBV transformationIgG1λ2IC50 < 100 ng/mL[56]
8B10HumanCHIKVE1-E2E2 Domain AEBV transformationIgG1kIC50 < 100 ng/mL

CHK-152MurineCHIKVE2aa 59HybridomaIgG2cIC50 1–3 ng/mL[57]

11E7MurineCCHFVGnC-terHybridomaIgGPRNT80 diluted 1/2560[58]

4-39-CCMurineRVFVG2Domain IVHybridomaIgGPRNT80 diluted 1/20480–1/81920[59]

2C9MurineYFVEHybridomaIgG2aPRNT90: 
1/104 17D
1/105.2 Asibi
[60]

5A
7A
R3(27)
HumanYFVEDI-DIIPhage displayScFvPRNT90:
0.1–0.3 μg/mL
[61]

13D6MurineTBEVEDIIIHybridomaIgG/chimericPRNT50: 1.9 μg/mL
FRNT50: 4.5 μg/mL
IC50
1.9–16.7 μg/mL
[62]

3B4C-4
(Hy4-26C)
MurineVEEVE2aa 182–209HybridomaIgG/humanizedPRNT70 (μg/mL):  
39.4–125
[63]