Figure 3: Effect of rotenone on alpha-synuclein (asyn), MEF2D, lamp2A, and hsc70 protein expression. (a) Representative Western blot analysis of asyn, MEF2D, lamp2A, and hsc70 in SH-SY5Y cells in basal conditions and after exposure to 200 nM rotenone for 24 hours. The immunoreactivity of beta-actin, used as internal standard, was also shown. (b) Mean values of asyn, MEF2D, lamp2A, and hsc70 protein expression, expressed as ratio between each target and beta-actin optical density. , paired Student’s -test; versus vehicle.